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FLASH GENE
Symbol CDC25B contributors: mct/npt/pgu - updated : 09-01-2014
HGNC name cell division cycle 25 homolog B (S. pombe)
HGNC id 1726
Location 20p13      Physical location : 3.776.400 - 3.786.759
Synonym name
  • cdc25 protein phosphatase, non receptor type, form B
  • cell division cycle 25 homolog B (S. cerevisiae)
  • dual specificity phosphatase Cdc25B
  • M-phase inducer phosphatase 2
  • EC.number 3.1.3.48
    DNA
    TYPE functioning gene
    STRUCTURE 19.35 kb     16 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    regulatory sequence Promoter
    Binding site
    text structure
  • TP53 binds to the CDC25B promoter and mediates transcriptional attenuation through the Sp1 and NF-Y transcription factors
  • MAPPING cloned Y linked   status confirmed
    Physical map
    AVP 20p13 arginine vasopressin (neurophysin II, antidiuretic hormone, diabetes insipidus, neurohypophyseal) UBCE7IP5 20p13 likely ortholog of mouse ubiquitin conjugating enzyme 7 interacting protein 5 FLJ13149 20p13 hypothetical protein FLJ13149 ProSAPiP1 20p13 ProSAPiP1 protein C20orf116 20p13 chromosome 20 open reading frame 116 ITPA 20p inosine triphosphatase (nucleoside triphosphate pyrophosphatase) SLC4A11 20p12 solute carrier family 4, sodium bicarbonate transporter-like, member 11 C20orf194 20p13 chromosome 20 open reading frame 194 UBE2V1P1 20p13 ubiquitin-conjugating enzyme E2 variant 1 pseudogene 1 ATRN 20p13 attractin SF3A3P 20p13 splicing factor 3a, subunit 3 pseudogene GFRA4 20p13-p12 GDNF family receptor alpha 4 ADAM33 20p13 a disintegrin and metalloproteinase domain 33 SN 20p13 sialoadhesin HSPA12B 20p13 heat shock 70kD protein 12B C20orf27 20p13 chromosome 20 open reading frame 27 C20orf28 20pter-q11.23 chromosome 20 open reading frame 28 CENPB 20p13 centromere protein B, 80kDa CDC25B 20p13 cell division cycle 25B C20orf29 20p13 chromosome 20 open reading frame 29 KIAA1271 20p13 KIAA1271 protein PANK2 20p13 pantothenate kinase 2 (Hallervorden-Spatz syndrome) RNF24 20p13-p12.1 ring finger protein 24 LOC388783 20 similar to hypothetical protein RPL21P2 20p13 ribosomal protein L21 pseudogene 2 MGC34919 20p13 hypothetical protein MGC34919 SMOX 20p13 spermine oxidase ADRA1D 20p13 adrenergic, alpha-1D-, receptor
    RNA
    TRANSCRIPTS type messenger
    text
  • the three isoforms seem to have a different level of activity
  • identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    16 splicing 3696 64.9 580 in spermatozoa 2007 18089784
  • CDC25B3
  • 14-3-3 preferentially binds to the motif Ser323
  • 16 splicing 3659 63.3 566 in spermatozoa 2007 18089784
  • CDC25B1
  • using an alternate splice site in the 5' coding region compared to variant 1
  • 14-3-3 preferentially binds to the motif at Ser309
  • 15 splicing 3578 60.6 539 in spermatozoa 2007 18089784
  • CDC25B2
  • lacking an alternate in-frame exon compared to variant 1
  • - - 2626 - 388 - 2007 18089784
    - - 2721 - 427 - 2007 18089784
    - - 2844 - 468 - 2007 18089784
    - - 3071 - 516 - 2007 18089784
    - - 3029 - 502 - 2007 18089784
    - - 2990 - 489 - 2007 18089784
    - - 2802 - 468 - 2007 18089784
    - - - - - - 2011 21363925
  • N-terminally truncated CDC25B isoforms, with an exclusively nuclear and nonredundant function in cell cycle re-initiation after DNA damage
  • previously unrecognized CDC25B isoform that operates specifically in the nucleus to reinitiate G2/M transition after DNA damage
  • EXPRESSION
    Type
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestiveintestinelarge intestinecolon moderately
     pancreas exocrine   moderately
     salivary gland   predominantly
    Lymphoid/Immunethymus   highly
     tonsils   moderately
    Nervousbrain   moderately
     nervecranial nerve  highly
    Reproductivefemale systemovary  moderately
     female systembreastmammary gland moderately
     female systemuterus  moderately
    Respiratorylung   moderately
    Skin/Tegumentskin   moderately
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Connectiveadipose  moderately
    cell lineage
    cell lines
    fluid/secretion moderately in blood
    at STAGE
    cell cycle     cell cycle, checkpoint, G2M
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a rhodanese homology domain
  • a nuclear localization signal (NLS)
  • a nuclear export signal
  • five 14-3-3 binding motifs
  • AA C-terminal to the 14-3-3 binding motif affect the efficiency of 14-3-3 binding
  • HOMOLOGY
    interspecies homolog to rattus Cdc25b (80.7 pc)
    homolog to murine Cdc25b (81.9 pc)
    Homologene
    FAMILY
  • MPI phosphatase family
  • CDC25 dual-specificity phosphatase family
  • CATEGORY enzyme , protooncogene
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
    intracellular,cytoplasm,cytoskeleton,microtubule,mitotic spindle
    intracellular,nucleus
    text
  • being nuclear in M and G1 phases
  • moving to the cytoplasm during S and G2
  • localizes to the centrosomes from early S phase
  • basic FUNCTION
  • dual specificity (tyr/thr) phosphatase activity
  • regulator of the cell cycle
  • functioning as a dosage-dependent inducer of mitotic progression
  • directly dephosphorylating CDC2 and stimulating its kinase activity
  • activating the cyclin dependent kinase CDC2 through dephosphorylation of T14 and Y15
  • playing a key role in the checkpoint response to DNA injury
  • having diverse noncell-cycle-related functions of CDC25B in terminally differentiated germ cells
  • coordinate cell cycle progression through activating dephosphorylation of Cyclin-dependent kinases
  • believed to trigger entry into mitosis
  • has an exclusive role in centrosome separation in late G2, and presumably acts as a trigger of mitotic onset
  • unique role in G2/M checkpoint control which is probably associated with existence of a family of splice variants with specific features controlling their localisation and activity
  • plays a critical role in the control of the cell cycle and in the checkpoint response to DNA damage
  • function as key players in G2/M cell cycle progression by activating the CDK1-cyclinB1 complexes
  • with CSC25A and CDC25C, are implicated at G1/S, during S-phase, at G2/M and during mitosis (are implicated at G1/S, during S-phase, at G2/M and during mitosis
  • its expression in neural progenitors has two functions: to change cell cycle kinetics and in particular G2-phase length and also to promote neuron production, identifying new roles for this phosphatase during neurogenesis
  • CDC25B and CDC25C play a major role in G2/M progression, whereas CDC25A assists in G1/S transition
  • CELLULAR PROCESS cell cycle, division, mitosis
    cell life, differentiation
    cell life, proliferation/growth
    PHYSIOLOGICAL PROCESS reproduction/sex
    text
  • positive control of cell proliferation
  • oocyte maturation
  • PATHWAY
    metabolism
    signaling
    part of the pathway that controls the localization of gamma-tubulin to the centrosomes, thereby regulating centrosome duplication during S phase and the nucleation of microtubules
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • interacting with PLK1 (PLK1 regulates CDC25B-dependent mitosis entry)
  • SEPW1 activated CDC25B by promoting the dissociation of 14-3-3 from CDC25B through the reduction of the intramolecular disulfide bond during recovery
  • RPS6KA3 preferentially phosphorylates CDC25A and CDC25B in mitotic cells
  • through activation of a centrosomal pool of CDK2, stabilises the local pool of Monopolar spindle 1 (Mps1) which in turn regulates the level of CETN2 at the centrosome
  • cell & other
    REGULATION
    activated by stimulated by cyclins B
    Phosphorylated by CDK1/CyclinB on at least 11 SP or TP sites, and 10 of these sites are also phosphorylated by CDK/Cyclins
    Other phosphorylated and inactivated by CHEK1
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    constitutional     --over  
    caused an earlier centrosome splitting in late G2
    tumoral     --over  
    in a significant number of cancers, including colon carcinoma, and often associated with high grade tumours and poor prognosis
    constitutional     --low  
    results in a specific lengthening of the G2 phase, whereas the S-phase length and the total cell cycle time are not significantly modified
    tumoral     --over  
    is commonly found in human cancer, results in a significant increase in centrin 2 at the centrosomes of interphase cells
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    SystemTypeDisorderPubmed
    cancer  
    expression level of CDC25B might be a potential key parameter of the cellular response to cancer therapy
    ANIMAL & CELL MODELS
  • Cdc25b-deficient mice