Selected-GenAtlas references SOURCE GeneCards NCBI Gene Swiss-Prot Ensembl
HGNC UniGene Nucleotide OMIM UCSC
Home Page
FLASH GENE
Symbol CDC25B contributors: mct/npt/pgu - updated : 09-01-2016
HGNC name cell division cycle 25 homolog B (S. pombe)
HGNC id 1726
Location 20p13      Physical location : 3.776.400 - 3.786.759
Synonym name
  • cdc25 protein phosphatase, non receptor type, form B
  • cell division cycle 25 homolog B (S. cerevisiae)
  • dual specificity phosphatase Cdc25B
  • M-phase inducer phosphatase 2
  • EC.number 3.1.3.48
    DNA
    TYPE functioning gene
    STRUCTURE 19.35 kb     16 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    regulatory sequence Promoter
    Binding site
    text structure
  • TP53 binds to the CDC25B promoter and mediates transcriptional attenuation through the Sp1 and NF-Y transcription factors
  • MAPPING cloned Y linked   status confirmed
    RNA
    TRANSCRIPTS type messenger
    text
  • the three isoforms seem to have a different level of activity
  • identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    16 splicing 3696 64.9 580 in spermatozoa 2007 18089784
  • CDC25B3
  • 14-3-3 preferentially binds to the motif Ser323
  • 16 splicing 3659 63.3 566 in spermatozoa 2007 18089784
  • CDC25B1
  • using an alternate splice site in the 5' coding region compared to variant 1
  • 14-3-3 preferentially binds to the motif at Ser309
  • 15 splicing 3578 60.6 539 in spermatozoa 2007 18089784
  • CDC25B2
  • lacking an alternate in-frame exon compared to variant 1
  • - - 2626 - 388 - 2007 18089784
    - - 2721 - 427 - 2007 18089784
    - - 2844 - 468 - 2007 18089784
    - - 3071 - 516 - 2007 18089784
    - - 3029 - 502 - 2007 18089784
    - - 2990 - 489 - 2007 18089784
    - - 2802 - 468 - 2007 18089784
    - - - - - - 2011 21363925
  • N-terminally truncated CDC25B isoforms, with an exclusively nuclear and nonredundant function in cell cycle re-initiation after DNA damage
  • previously unrecognized CDC25B isoform that operates specifically in the nucleus to reinitiate G2/M transition after DNA damage
  • EXPRESSION
    Type
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestiveintestinelarge intestinecolon moderately
     pancreas exocrine   moderately
     salivary gland   predominantly
    Lymphoid/Immunethymus   highly
     tonsils   moderately
    Nervousbrain   moderately
     nervecranial nerve  highly
    Reproductivefemale systemovary  moderately
     female systembreastmammary gland moderately
     female systemuterus  moderately
    Respiratorylung   moderately
    Skin/Tegumentskin   moderately
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Connectiveadipose  moderately
    cell lineage
    cell lines
    fluid/secretion moderately in blood
    at STAGE
    cell cycle     cell cycle, checkpoint, G2M
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a rhodanese homology domain
  • a nuclear localization signal (NLS)
  • a nuclear export signal
  • five 14-3-3 binding motifs
  • AA C-terminal to the 14-3-3 binding motif affect the efficiency of 14-3-3 binding
  • HOMOLOGY
    interspecies homolog to rattus Cdc25b (80.7 pc)
    homolog to murine Cdc25b (81.9 pc)
    Homologene
    FAMILY
  • MPI phosphatase family
  • CDC25 dual-specificity phosphatase family
  • CATEGORY enzyme , protooncogene
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
    intracellular,cytoplasm,cytoskeleton,microtubule,mitotic spindle
    intracellular,nucleus
    text
  • being nuclear in M and G1 phases
  • moving to the cytoplasm during S and G2
  • localizes to the centrosomes from early S phase
  • basic FUNCTION
  • dual specificity (tyr/thr) phosphatase activity
  • regulator of the cell cycle
  • functioning as a dosage-dependent inducer of mitotic progression
  • directly dephosphorylating CDC2 and stimulating its kinase activity
  • activating the cyclin dependent kinase CDC2 through dephosphorylation of T14 and Y15
  • playing a key role in the checkpoint response to DNA injury
  • having diverse noncell-cycle-related functions of CDC25B in terminally differentiated germ cells
  • coordinate cell cycle progression through activating dephosphorylation of Cyclin-dependent kinases
  • believed to trigger entry into mitosis
  • has an exclusive role in centrosome separation in late G2, and presumably acts as a trigger of mitotic onset
  • unique role in G2/M checkpoint control which is probably associated with existence of a family of splice variants with specific features controlling their localisation and activity
  • plays a critical role in the control of the cell cycle and in the checkpoint response to DNA damage
  • function as key players in G2/M cell cycle progression by activating the CDK1-cyclinB1 complexes
  • with CSC25A and CDC25C, are implicated at G1/S, during S-phase, at G2/M and during mitosis (are implicated at G1/S, during S-phase, at G2/M and during mitosis
  • its expression in neural progenitors has two functions: to change cell cycle kinetics and in particular G2-phase length and also to promote neuron production, identifying new roles for this phosphatase during neurogenesis
  • CDC25B and CDC25C play a major role in G2/M progression, whereas CDC25A assists in G1/S transition
  • CELLULAR PROCESS cell cycle, division, mitosis
    cell life, differentiation
    cell life, proliferation/growth
    PHYSIOLOGICAL PROCESS reproduction/sex
    text
  • positive control of cell proliferation
  • oocyte maturation
  • PATHWAY
    metabolism
    signaling
    part of the pathway that controls the localization of gamma-tubulin to the centrosomes, thereby regulating centrosome duplication during S phase and the nucleation of microtubules
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • interacting with PLK1 (PLK1 regulates CDC25B-dependent mitosis entry)
  • SEPW1 activated CDC25B by promoting the dissociation of 14-3-3 from CDC25B through the reduction of the intramolecular disulfide bond during recovery
  • RPS6KA3 preferentially phosphorylates CDC25A and CDC25B in mitotic cells
  • through activation of a centrosomal pool of CDK2, stabilises the local pool of Monopolar spindle 1 (Mps1) which in turn regulates the level of CETN2 at the centrosome
  • PTBP1 controls mRNA abundance and alternative splicing of important cell cycle regulators including CCND2, MYC, RBL1 and CDC25B
  • cell & other
    REGULATION
    activated by stimulated by cyclins B
    Phosphorylated by CDK1/CyclinB on at least 11 SP or TP sites, and 10 of these sites are also phosphorylated by CDK/Cyclins
    Other phosphorylated and inactivated by CHEK1
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    constitutional     --over  
    caused an earlier centrosome splitting in late G2
    tumoral     --over  
    in a significant number of cancers, including colon carcinoma, and often associated with high grade tumours and poor prognosis
    constitutional     --low  
    results in a specific lengthening of the G2 phase, whereas the S-phase length and the total cell cycle time are not significantly modified
    tumoral     --over  
    is commonly found in human cancer, results in a significant increase in centrin 2 at the centrosomes of interphase cells
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    SystemTypeDisorderPubmed
    cancer  
    expression level of CDC25B might be a potential key parameter of the cellular response to cancer therapy
    ANIMAL & CELL MODELS
  • Cdc25b-deficient mice