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Symbol BTAF1 contributors: mct/npt - updated : 08-07-2020
HGNC name BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae)
HGNC id 17307
Location 10q23.32      Physical location : 93.683.735 - 93.790.078
Synonym name
  • TATA box binding protein (TBP) associated factor, RNA polymerase 2D
  • TBP associated factor 172
  • ATP-dependent helicase BTAF1
  • modifier of transcription 1
  • Synonym symbol(s) MOT1, TAF172, TAF-172, TAFII170, TAF2D2, MGC138406, KIAA0940
    EC.number 3.6.1.-
    DNA
    TYPE like-sequence
    SPECIAL FEATURE arranged in tandem
    STRUCTURE 106.35 kb     38 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    regulatory sequence alternative promoter
    motif
    text structure multiple transcription start sites
    MAPPING cloned Y linked N status provisional
    regionally located located between D10S185 and WI-1183
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    38 - 7054 206.7 1849 - 2000 10642510
    EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestiveliver    
    Endocrinepancreas    
    Lymphoid/Immunethymus   highly
    Nervousbrain    
    Reproductivemale systemprostate   
    Respiratoryrespiratory tracttrachea  highly
    Urinarykidney    
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Blood / Hematopoieticbone marrow   
    Connectivebone   
    Epithelialsecretoryglandularendocrine 
    Lymphoid    
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • . an amino-terminal TBP binding region of approximately 90140&
  • 8201;kDa with predicted HEAT repeats
  • nuclear localization signal (NLS)
  • DEAD/DEAH (Asp-Glu-Ala-Asp/His) box
  • helicase conserved C-terminal domain, with Swi2/Snf2-type ATPase domain
  • HOMOLOGY
    interspecies homolog to yeast S.cerevisiae Mot1
    homolog to Drosophila Tis11
    Homologene
    FAMILY
  • SNF2/RAD54 helicase family
  • SNF2/SWI2 family of DNA targeted ATPases
  • CATEGORY transcription factor
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,nucleus,nucleoplasm
    basic FUNCTION
  • inhibiting TBP-driven RNA polymerase II and III transcription
  • removing TBP from DNA (TATA box) in an ATP-dependent manner
  • ATP-dependent helicase regulating TBP function on POLII promoters in cells
  • Swi2/Snf2 enzyme that specifically displaces TBP from the promoter DNA and regulates transcription globally by generating a highly dynamic TBP pool in the cell
  • TBP, BTAF1, and DR1 establish a regulatory circuit that controls downstream core promoter element-dependent versus TATA-dependent transcription b
  • negatively regulates TBP and DR1 binding to active promoters
  • not only displaces TBP, but blocks its hydrophobic surface patch to prevent interactions with DNA or other factors that bind to the concave surface
  • CELLULAR PROCESS nucleotide, transcription, regulation
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • component of the B-TFIID complex with TBP
  • INTERACTION
    DNA binding
    RNA
    small molecule nucleotide,
  • ATP
  • protein
  • binding to TBP
  • action of BTAF1 is required to clear TBP from intrinsically preferred (TATA-containing) binding sites, ensuring sufficient soluble TBP to bind intrinsically disfavored (TATA-less) sites
  • TBP-DNA affinity as well as other aspects of promoter sequence influence BTAF1 function
  • lifetime of TBP at the promoter site is controlled by several cofactors including the Modifier of transcription 1 (BTAF1)
  • O-GlcNAcylation at T114 of TBP blocks its interaction with BTAF1, hence the formation of the B-TFIID complex, and its dynamic cycling on and off of DNA
  • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS