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FLASH GENE
Symbol ARFGEF1 contributors: mct - updated : 10-04-2014
HGNC name ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
HGNC id 15772
Location 8q13.2      Physical location : 68.109.896 - 68.255.912
Synonym name
  • brefeldin A-inhibited guanine nucleotide exchange protein 1, for ADP-ribosylation factor, predominantly toward ARF1 and ARF5
  • Arf guanine nucleotide exchange factor
  • Synonym symbol(s) BIG1, P200, ARFGEP1
    DNA
    TYPE functioning gene
    STRUCTURE 170.18 kb     39 Exon(s)
    MAPPING cloned Y linked N status provisional
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    6 - 4735 - 1221 - 2005 15644318
    EXPRESSION
    Type widely
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Cardiovascularheart    
    Digestiveintestinelarge intestinecolon highly
    Endocrineparathyroid   highly
    Urinarykidney    
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a SEC7 domain characteristic of ARF gamma nucleotide exchange proteins
  • C-terminal region including a binding site for myosin IXb
  • HOMOLOGY
    Homologene
    FAMILY Sec7 family of ADP-ribosylation factor (ARF) guanine nucleotide-exchange proteins (GEP)
    CATEGORY regulatory , transport
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,organelle,Golgi
    intracellular,cytoplasm,cytosolic
    intracellular,nucleus,nucleoplasm
    intracellular,nucleus,nucleolus
    intracellular,nuclear envelope
    text
  • cytosolic and colocalizing with COPB1 in a perinuclear structure probably with Golgi membranes
  • GBF1 acts at the cis-Golgi, whereas ARFGEF1 and its close paralog ARFGEF2 act at the trans-Golgi
  • basic FUNCTION
  • activators of ADP-ribosylation factor GTPases that are essential for regulating vesicular traffic among intracellular organelles
  • preferentially activates ADP-ribosylation factor 1 (ARF1) and ARF3
  • also involved in nuclear functions
  • required for correct glycosylation and function of integrin beta1 and playing a role in cytoskeletal dynamics through integrin-beta1-mediated signaling
  • ARFGEF1, ARFGEF2 play redundant roles in trafficking between the TGN and endosomes that involves the AP-1 complex
  • ARFGEF1 is required to maintain the normal morphology of the Golgi but ARFGEF2 is important for endosomal compartment integrity and cannot replace the function of ARFGEF1 in Golgi organization
  • effects of ARFGEF1 and KANK1 proteins on cell polarity and directed migration during wound healing
  • Arf guanine nucleotide-exchange factors ARFGEF1, ARFGEF2 and regulate nonmuscle myosin IIA activity by anchoring myosin phosphatase complex
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling signal transduction
    a component
  • component with ARFGEF2 of a macromolecular complex
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • interacting with ARF proteins
  • binding partner for myosin IXb, which inhibited the GAP activity of myosin IXb (MYO9B)
  • interacting with KIF21A
  • ARL1 is necessary for Golgi recruitment of ARFGEF1 and ARFGEF2 but not GBF1
  • through its ability to activate ADP-ribosylation factor 1, regulates cell-surface levels and function of ABCA1, indicating a transcription-independent mechanism for controlling ABCA1 action
  • GBF1-activated ARFs (ARF4 and ARF5, but not ARF3) facilitate ARFGEF1 and ARFGEF2 recruitment to the trans-Golgi network (TGN)
  • cell & other
    REGULATION
    inhibited by brefeldin A
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS