Selected-GenAtlas references SOURCE GeneCards NCBI Gene Swiss-Prot Ensembl
HGNC UniGene Nucleotide OMIM UCSC
Home Page
FLASH GENE
Symbol APOBEC3F contributors: mct - updated : 10-02-2016
HGNC name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F
HGNC id 17356
Location 22q13.1      Physical location : 39.436.672 - 39.451.973
Synonym name
  • induced upon T-cell activation
  • cytidine-to-uridine RNA-editing deaminase
  • APOBEC-related cytidine deaminase
  • DNA dC->dU-editing enzyme APOBEC-3G
  • Synonym symbol(s) ARP8, KA6, MGC74891, CEM15, BK150C2.4.MRNA, A3F
    EC.number 3.5.4.-
    DNA
    TYPE functioning gene
    SPECIAL FEATURE arranged in tandem, component of a cluster
    text cluster of APOBEC1-like genes and pseudogenes on chromosome 22
    STRUCTURE 15.31 kb     7 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    regulatory sequence Promoter
    Binding site
    MAPPING cloned Y linked N status provisional
    Physical map
    KIAA0063 22q13.1 platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) GTPBP1 22q13.1 GTP binding protein 1 UNC84B 22q13.1-q13.3 unc-84 homolog B (C. elegans) DNAL4 22q13.1 dynein, axonemal, light polypeptide 4 LOC388901 22 LOC388901 NPTXR 22q13.1 neuronal pentraxin receptor CBX6 22q13.1 chromobox homolog 6 APOBEC3A 22q13.1-q13.2 apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A APOBEC3B 22q12-q13 apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B APOBEC3C 22q12-q13 apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3C APOBEC3D 22q12-q13 apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3D APOBEC3E 22q12-q13 apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3E pseudogene APOBEC3F 22q12-q13 apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F APOBEC3G 22q12-q13 apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G ARP10 22q13.1 ARP10 protein COX5BL7 22pter-p13 cytochrome c oxidase subunit Vb-like 7 CBX7 22q13.1 chromobox homolog 7 LOC391332 22 similar to hepatitis C virus core-binding protein 6; cervical cancer oncogene 3 FLJ23865 22q13.1 hypothetical protein FLJ23865 PDGFB 22q13.1 platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    7 - 4706 45 373 - 2007 17522216
    3 - 822 - 101 - 2007 17522216
    6 - - 37 - prominently expressed in macrophages and monocytes 2010 20624919
  • variant lacking exon 2
  • resistant to Vif-mediated degradation
  • 4 - - 24 184 - 2010 20624919
  • lacks exons 2-4
  • highly sensitive to Vif
  • EXPRESSION
    Type widely
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestiveliver   highly
    Endocrinepancreas   highly
     parathyroid   highly
    Lymphoid/Immunelymph node   highly
     tonsils   highly
    cells
    SystemCellPubmedSpeciesStageRna symbol
    Lymphoid/ImmuneT cell Homo sapiens
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • N-terminal cytidine deaminase active site with an insert,
  • a linker region and a pseudoactive site
  • conserved zinc-binding ligands
  • an active-site glutamate required for proton shuttling
  • 2 aromatic residues required for RNA binding
  • C-terminal half is a duplication of the active site, insert, and linker region, with Vif-interacting domain, domain that interacts with the Vif DRMR region located between AAs 283 and 300 , and a cytidine deaminase motif, located in homologous N-terminal and C-terminal domains
  • dual conserved catalytic domains located in the N- and C-terminal regions termed CD1 and CD2, respectively
  • Glu324 is a surface AA within the alpha4 helix adjacent to AAs corresponding to other known Vif susceptibility determinants in APOBEC3G and APOBEC3H
  • HOMOLOGY
    Homologene
    FAMILY
  • cytidine deaminase family
  • CATEGORY RNA associated
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm
    basic FUNCTION
  • functioning as antiretroviral DNA-editing enzyme
  • inhibiting replication of Vif-deficient human immunodeficiency virus type 1 (HIV-1)
  • mediates intrinsic resistance of monocyte-derived dendritic cells to HIV-1 infection
  • APOBEC3G and APOBEC3F are potent inhibitors of retroviruses
  • restrict retroviral infection by deaminating cytosine residues in the first cDNA strand of a replicating virus
  • APOBEC3F and APOBEC3G inhibit HIV-1 DNA integration by different mechanisms
  • have strong antiviral activity and together with APOBEC3G, it is considered the most potent cytidine deaminase targeting HIV
  • potently restricts the infectivity of HIV-1 in the absence of the viral accessory protein virion infectivity factor (Vif)
  • APOBEC3F and APOBEC3G are the most potent inhibitors of HIV-1, but only in the absence of the virus-encoded protein, Vif
  • antiretroviral activity of cellular proteins APOBEC3F and APOBEC3G requires their inclusion within HIV-1 virions
  • APOBEC3F (A3F) and APOBEC3G (A3G) inhibit human immunodeficiency virus type-1 (HIV-1) replication
  • unlike APOBEC3G, signals in N- and C-terminal deaminase domains of APOBEC3F each contribute to virion encapsidation
  • APOBEC3F, APOBEC3G are host factors that incorporate into virions and restrict virus replication
  • catalytic activity of APOBEC3F is required for efficient restriction of Vif-deficient human immunodeficiency virus
  • incorporation of APOBEC3F into virions is a prerequisite for exerting its antiviral function
  • APOBEC3F and APOBEC3G are two of the most potent A3 enzymes in humans with each having a different target DNA specificity: A3G prefers to deaminate cytosines preceded by a cytosine (5'-CC), whereas A3F preferentially targets cytosines preceded by a thymine (5'-TC)
  • APOBEC3 proteins function within the innate immune system by mutating DNA of viral genomes and retroelements to restrict infection and retrotransposition
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • Vif protein of lentiviruses (to induce its degradation by proteasomes)
  • conserved motif VxIPLx(4-5)LxPhix(2)YWxL motif in HIV-1 Vif required for APOBEC3G and APOBEC3F interaction and inhibition
  • HIV-1 Vif targets cellular antiviral APOBEC3F (A3F) enzyme for degradation
  • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    ANIMAL & CELL MODELS