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FLASH GENE
Symbol AJUBA contributors: mct - updated : 21-02-2017
HGNC name ajuba LIM protein
HGNC id 20250
Location 14q11.2      Physical location : -
Synonym name
  • protein ajuba
  • jub, ajuba homolog (Xenopus laevis)
  • Synonym symbol(s) MGC15563, JUB
    DNA
    TYPE functioning gene
    STRUCTURE 11.47 kb     8 Exon(s)
    MAPPING cloned Y linked N status provisional
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    8 - 4236 - 538 - 2006 16713569
    6 - 3263 - 121 - 2006 16713569
    3 - 1807 - 369 - 2006 16713569
    EXPRESSION
    Type widely
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestiveliver   highly
     mouthtongue  highly
    Reproductivefemale systemuteruscervix highly
    Skeletonaxialskullfacemandible 
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Muscularstriatumskeletal  
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    physiological period embryo, fetal
    Text expressed in totipotent embryonic stem cells, in the embryonic yolk sac, and in the fetal liver
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • three LIM zinc-binding domains, with a PreLIM domain
  • HOMOLOGY
    Homologene
    FAMILY
  • zyxin/ajuba family
  • Ajuba LIM proteins family
  • CATEGORY adaptor , transport
    SUBCELLULAR LOCALIZATION     plasma membrane,junction,adherens
        intracellular
    intracellular,cytoplasm,organelle,Golgi
    intracellular,cytoplasm,cytosolic
    intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
    intracellular,nucleus,nucleoplasm,nuclear bodies
    text
  • associates with the actin cytoskeleton near the adhesion plaques
  • localizes to basal bodies of cilia in growth-arrested cells
  • cytoplasmic protein that can shuttle into the nucleus (Hou 2010)
  • basic FUNCTION
  • may be a component of a signal transduction pathway mediating adhesion-stimulated changes in gene expression
  • may be contributing to the bridging of the cadherin adhesive complexes to the actin cytoskeleton and to the formation of cadherin-mediated cell-cell adhesion
  • function as corepressors for SNAI1 and, as such, may serve as a platform for the assembly of chromatin-modifying factors
  • having a role in the regulation of vertebrate ciliogenesis and left-right axis determination
  • functions as a nuclear receptor corepressor and negatively regulates retinoic acid signaling
  • adaptor protein that exhibit the potential to communicate cell adhesive events with nuclear responses to remodel epithelia
  • having role as negative regulatorof the Hippo signaling pathway
  • required for microRNA-mediated gene silencing
  • crucial regulator of the second heart field (SHF) progenitor cell specification and expansion
  • AJUBA protects against unscheduled ATR signaling by preventing inappropriate RPA1 phosphorylation
  • required for MAPK8-mediated activation of YAP1 and AJUBA contributed to wing regeneration after wounding and to tumor growth
  • negatively regulates YAP1 activity through the LATS family, and inactivation of AJUBA is a novel key mechanism in malignant mesothelioma cell proliferation
  • is an important regulator of adipocyte differentiation by functioning as an obligate co-activator of PPARG
  • is a novel coactivator for NR1H2, NR1H3 and may play important role in lipid and glucose metabolism
  • AJUBA LIM proteins limit Hippo pathway activity in contexts where cell proliferation is needed
  • is a scaffold protein participating in the regulation of cell adhesion, mitosis, DNA damage, cell differentiation, proliferation, migration and gene transcription
  • AJUBA can promote colorectal cancer growth via inhibiting apoptosis
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • component at cadherin-mediated cell-cell junction
  • AKT1-PAK1-AJUBA feedback loop integrates spatiotemporal signaling with actin remodeling at cell-cell contacts and stabilizes preassembled cadherin complexes
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • binding AURKA in mitotic cells
  • corepressor of GFI1 (SNAG domain of GFI1 may bind Ajuba, trapping it in the nucleus where it functions as an adapter or molecular scaffold for the assembly of macromolecular repression complexes at target promoters)
  • directly interacts with RARA via its conserved nuclear-receptor binding motifs in a ligand-dependent manner
  • bound directly to AKT1·GDP or AKT1·GTP, but phosphorylated AJUBA interacted preferentially with active AKT1
  • AJUBA binds ISL1, represses its transcriptional activity, and is also required for autorepression of ISL1 expression in an Retinoic acid (RA)-dependent manner
  • PreLIM domain of AJUBA, an important activator of AURKA, induces the autophosphorylation of the C-terminal kinase domain of AURKA, and is phosphorylated by the C-terminal domain of AURKA
  • associates with CTNNA1 and its localization to adherens junctions and association with CTNNA1 are promoted by cytoskeletal tension
  • recruits LATS1 to junctions in a tension-dependent manner
  • recruits CREBBP via its LIM domain and facilitates CREBBP binding to PPARG
  • binds to the hinge and the ligand binding domains of NR1H3 via its C-terminal tandem LIM motifs and enhances NR1H3 target gene expression in liver cells
  • AJUBA represses the expression of IFIT2 gene, an interferon-stimulated gene and a known apoptosis inducer and tumour suppressor to mediate its resistance to apoptosis
  • cell & other
    REGULATION
    Phosphorylated by PAK1, that transiently activated upon cell-cell adhesion and directly phosphorylated AJUBA (Thr172)
    is phosphorylated by cyclin-dependent kinase 1 (CDK1) at Ser(119) and Ser(175) during the G2/M phase of the cell cycle
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    tumoral     --over  
    in colorectal cancer (CRC)
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
  • marker for diagnosis of CRC
  • Therapy target
    SystemTypeDisorderPubmed
    cancerdigestivecolon
    target for the therapeutics of CRC
    ANIMAL & CELL MODELS
  • Ajuba-deficient zebrafish embryos show an increased pool of Isl1(+) cardiac progenitors and, subsequently, dramatically increased numbers of cardiomyocytes at the arterial and venous poles