Selected-GenAtlas references SOURCE GeneCards NCBI Gene Swiss-Prot Ensembl
HGNC UniGene Nucleotide OMIM UCSC
Home Page
FLASH GENE
Symbol ZW10 contributors: mct/npt - updated : 17-12-2014
HGNC name ZW10, kinetochore associated, homolog (Drosophila)
HGNC id 13194
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • kinetochore localization domain is the ZWINT interaction domain (Famulski 2008)
  • C-terminal region interacts with dynamitin (DCTN2), and part of this region has been used as an inhibitor of ZW10 function (Inoue 2008)
  • HOMOLOGY
    interspecies homolog to Drosophila zeste-white 10
    Homologene
    FAMILY
  • ZW10 family
  • CATEGORY regulatory , DNA associated
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,organelle,membrane
    intracellular,cytoplasm,organelle,endoplasmic reticulum
    intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
    intracellular,cytoplasm,cytoskeleton,microtubule,mitotic spindle
    intracellular,nucleus,chromatin/chromosome,centromere
    intracellular,nucleus,chromatin/chromosome,kinetochore
    text
  • moving from the centromere/kinetochore at prometaphase to kinetochore microtubules at metaphase and then back to the centromere/kinetochore at anaphase
  • kinetochore residency at prometaphase kinetochores is dependent on its interaction with ZWINT, and is essential for mitotic checkpoint arres (Famulski 2008)
  • located in the endoplasmic reticulum as well as in the cytosol during interphase (Hirose 2004)
  • ZWINT localizes to the kinetochore in prophase, before ZW10 localization, and remains at the kinetochore until anaphase, after ZW10 has dissociated
  • basic FUNCTION
  • centromere/kinetochore protein, recruiting dynein and dynactin to the centromere
  • functions as a linker between the core structural elements of the outer kinetochore and components that catalyze generation of the mitotic checkpoint-derived "stop anaphase" inhibitor
  • ZW10 and KNTC1 play an essential role in mitotic checkpoint
  • ZW10 and KNTC1 proteins are part of a complex necessary for accurate chromosome segregation
  • involved in membrane trafficking between the endoplasmic reticulum and Golgi.and playing a role during interphase (Hirose 2007)
  • implicated in anchoring of MAD1L1/MAD1L2 to the kinetochore and appears to also move off the kinetochore with MAD2L1 (Sivaram 2009)
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis
  • member of the RZZ complex (Rod–Zw10–Zwilch) (Sivaram 2009)
  • forms a subcomplex with RINT1 (Rad50-interacting protein) and p31 in a large complex comprising syntaxin 18, an endoplasmic reticulum-localized t-SNARE implicated in membrane trafficking (Hirose 2004)
  • RZZ (KNTC1/ZW10/ZWILCH) complex is required for the recruitment of cytoplasmic dynein to kinetochores
  • part of NRZ complex, which comprises KNTC1, RINT1, and ZW10, is also involved in Golgi-to-ER retrograde transport, but each component of the complex has diverse cellular functions including endosome-to-Golgi transport, cytokinesis, cell cycle checkpoint, autophagy, and mRNA decay
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • interacting with ZWINT (specifies the kinetochore association of ZW10 by interacting with its N-terminal domain)
  • weak interaction between the ZW10/KNTC1/ZWILCH complex and the kinesin-like kinetochore component CENPE
  • ZWINT is critical for recruiting ZW10 to unattached kinetochores
  • AURKB activity is required for the accumulation of tension-sensitive mitotic-checkpoint components, such as ZW10 and KNTC1, in order to maintain mitotic-checkpoint arrest
  • interacts with dynamitin, a subunit of the dynein accessory complex dynactin, and functions in termination of the spindle checkpoint during mitosis and in membrane transport between the endoplasmic reticulum (ER) and Golgi apparatus during interphase (Inoue 2008)
  • ZW10 kinetochore residency at prometaphase kinetochores is dependent on its interaction with ZWINT, and is essential for mitotic checkpoint arrest
  • NBAS links between USE1 and ZW10-RINT1 and is involved in Golgi-to-ER transport
  • CCDC99 is a cell cycle-regulated mitotic phosphoprotein that interacts with the KNTC1/ZW10/ZWILCH (RZZ) complex
  • PIAS4 interacts with the KNTC1/ZW10 complex
  • ZWINT, a kinetochore protein interact with the kinetochore checkpoint protein ZW10
  • RINT1 mediates the association of ZW10 (mammalian Dsl1) with endoplasmic reticulum-localized SNARE proteins
  • cell & other
    REGULATION
    Other regulated by RAB6 (both ZW10/RINT1 and conserved oligomeric Golgi complex-dependent Golgi trafficking and homeostasis) (Sun 2007)
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS