Selected-GenAtlas references SOURCE GeneCards NCBI Gene Swiss-Prot Ensembl
HGNC UniGene Nucleotide OMIM UCSC
Home Page
FLASH GENE
Symbol WEE1 contributors: mct/ - updated : 02-07-2016
HGNC name WEE1 homolog (S. pombe)
HGNC id 12761
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • N-terminal 214 residues facilitating its ubiquitin-dependent degradation to trigger mitotic entry
  • a protein kinase domain
  • HOMOLOGY
    interspecies homolog to yeast wee1
    homolog to murine Wee1
    Homologene
    FAMILY
  • protein kinase superfamily
  • SER/THR family of protein kinases
  • WEE1 subfamily
  • CATEGORY enzyme , regulatory
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm
    intracellular,nucleus,nucleoplasm
    basic FUNCTION
  • negative regulator of cell cycle
  • phosphorylation cyclin dependent kinases
  • insuring the completion of DNA replication prior to mitosis
  • playing a critical role in controlling the transition into mitosis when it involved in the Swe1-Cdk1 complex
  • inhibiting mitosis entry by phosphorylating cyclin B1/CDC2 (M phase promoting factor (MPF))
  • repression of WEE1 by Kruppel-like factor 2 (KLF2) is involved in DNA damage-induced apoptosis
  • cell-cycle checkpoint kinase that regulates the entry into mitosis in dividing cells
  • plays a key role in cell cycle progression at the onset of mitosis by phosphorylating CDK1 at the inhibitory Tyr15 AA residue
  • major regulator of the G(2) checkpoint in glioblastoma cells
  • protein kinase that negatively regulates mitotic entry in G2 phase by suppressing cyclin B-CDC2 activity
  • regulator of genomic stability
  • specifically protects the stability of stalled replication forks
  • may have important role in hypoxia- induced pathophysiological situations in endothelial cells
  • cell-cycle checkpoint regulator
  • CELLULAR PROCESS cell cycle, division, mitosis
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA
    RNA
    small molecule nucleotide,
  • ATP
  • protein
  • phosphorylating Y15 in CDC2 and other CDKs
  • CDK1
  • regulation of WEE1 by BRSK2 and BRSK1 is essential for the differentiation of polarized neurons
  • interacting with CSNK2B (participates in PLK1-WEE1 complex formation interacting directly with WEE1 and thereby contributing to the regulation of G2-M cell cycle transition)
  • USP50 may act through a HSP90AA1-dependent mechanism to counteract CDC25B mitotic inducing activity and prevent WEE1 degradation
  • WEE1 interacting with MUS81 (novel role of WEE1 in controlling MUS81 and DNA replication in human cells)
  • plays an important role in the regulation of WEE1 stability
  • CDC14A counteracts CDK1-CCNB1 activity through WEE1 dephosphorylation
  • both WEE1 and MYT1 can phosphorylate CDK1
  • ERRFI1 controls mitotic progression and the G2/M DNA damage checkpoint by stabilizing the WEE1 Kinase
  • cell & other
  • regulator of both HIV type 1 Vpr and gamma irradiation-induced apoptosis
  • REGULATION
    activated by transactivated by and increased in association with FOS/JUN (AP1)
    Cdk1 Phosphorylation
    inhibited by inactivated in phase M and G1 by autophosphorylation and protein degradation
    repressed by KLF2, by directly binding to an SP1/CPBP motif located between -252 bp and the start codon of the WEE1 promoter
    Phosphorylated by BRSK2, BRSK1 that are required to initiate its downregulation in polarized neurons
    Other regulation of its localization around the SPB (spindle pole body) during the G2/M transition is important for proper mitotic entry and progression
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    tumoral     --over  
    in hepatocellular carcinoma
    constitutional     --over  
    in rheumatoid cells inducing aberrant mitosis
    constitutional     --over  
    with PKMYT1 induced the phosphorylation of CDC2 leading to G2/M arrest
    constitutional     --low  
    caused a block in DNA replication, resulting in accumulation of cells in S phase
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    SystemTypeDisorderPubmed
    cancer  
    combination of WEE1 inhibitor and TNFSF10 could provide a novel combination for the treatment of basal/triple-negative breast cancer
    cancerbrainglioma/neuroblstoma
    inhibition of WEE1 kinase holds potential as a therapeutic approach in treatment of glioblastoma
    ANIMAL & CELL MODELS