Selected-GenAtlas references SOURCE GeneCards NCBI Gene Swiss-Prot Ensembl
HGNC UniGene Nucleotide OMIM UCSC
Home Page
FLASH GENE
Symbol KLF8 contributors: mct - updated : 22-02-2012
HGNC name Kruppel-like factor 8
HGNC id 6351
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • a PVDLS motif required for contact with GTBP1
  • two nuclear localization signals, one surrounding S165 and K171 and the other being two tandem ZFs, which are critical for the regulation of KLF8 nuclear localization and its cellular functions
  • a valine-rich hydrophobic stretch
  • an activation domain located between AAs 101-260 where the well-conserved Q118 and Q248 are essential for recruiting EP300 and KAT2B to activate target gene transcription
  • three highly conserved C2H2 zinc fingers on the C terminus, which can bind to the GT-box (CACCC) promoter sequence
  • conjugated sumoylated
    HOMOLOGY
    interspecies homolog to murine Klf8 (83.7 pc)
    Homologene
    FAMILY
  • Sp1 C2H2-type zinc-finger protein family
  • CATEGORY regulatory , transcription factor
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,nucleus
    basic FUNCTION
  • acting as a transcriptional silencer
  • being important in the regulation of the cell cycle
  • playing a critical role in oncogenic transformation and epithelial to mesenchymal transition
  • mediates cell cycle progression downstream of focal adhesion kinase (PTK2) by upregulating cyclin D1
  • required for v-Src-induced transformation and may play a role in tumor progression of cancer
  • regulates critical cellular processes including cell cycle progression, transformation, epithelial-to-mesenchymal transition, migration and invasion by either repressing or activating target gene promoters
  • critical inducer of epithelial to mesenchymal transition and invasion
  • its overexpression induced a strong increase in MMP9 expression and activity
  • regulates critical gene transcription and cellular events associated with cancer
  • CELLULAR PROCESS nucleotide, transcription, regulation
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA
  • binding to CACCC-boxes and GC boxes promoter elements
  • RNA
    small molecule metal binding,
  • Zn2+
  • protein
  • associating with C terminal binding proteins (CTBPs)and through the PVDLS motif
  • interacting with corepressor CtBP2
  • interacted with importin-beta and this interaction required the ZF motif
  • KLF8 activation domain located between AAs 101-260 where the well-conserved Q118 and Q248 are essential for recruiting EP300 and KAT2B to activate target gene transcription
  • interacting with MMP9 (KLF8 activation of MMP9 is a novel and critical signaling mechanism underlying human breast cancer invasion and metastasis)
  • PARP1 1s a novel KLF8-interacting protein and interaction is critical for maintaining the proper subcellular localization, transcription-regulating function, and protein stability of KLF8 in the nucleus
  • KLF8 promoted the EPSTI1 expression by directly acting on the EPSTI1 gene promoter
  • cell & other
    REGULATION
    induced by PTK2 in human ovarian cancer cells
    repressed by KLF3
    Other cellular function of KLF8 is regulated by post-translational sumoylation and acetylation
    sumoylation of KLF8 negatively regulates its transcriptional activity and cellular functions
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    constitutional   translocation --low  
    in a core of non syndromic mental retardation with translocation t(X;21)(p11.2;q22.3)
    tumoral        
    playing an important role in human breast cancer invasion and metastasis
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    SystemTypeDisorderPubmed
    cancer  
    co-targeting PARP1 and KLF8 (or other potential PARP1-interacting KLFs) as a novel anti-cancer therapeutic strategy
    ANIMAL & CELL MODELS