SUBCELLULAR LOCALIZATION
| intracellular
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| intracellular,cytoplasm,cytosolic
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| intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
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| intracellular,nucleus,nucleoplasm
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| intracellular,nucleus,chromatin/chromosome,kinetochore
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text
| localized at kinetochores, centrosomes, and the midbody at M phase |
basic FUNCTION
| plays a critical role in the early DNA-damage response |
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recruited by MDC1, through phosphodependent interactions between the RNF8 forkhead-associated domain and motifs in MDC1 that are phosphorylated by the DNA-damage activated protein kinase ataxia telangiectasia mutated (ATM) |
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the DNA-damage response is orchestrated by ATM-dependent phosphorylation of MDC1 and RNF8-mediated ubiquitination |
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facilitates the accumulation of checkpoint mediator proteins BRCA1 and TP53BP1 to the damaged chromatin |
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controls DNA damage-induced nuclear foci formation of PAXIP1, which in turn regulates TP53BP1 localization to the DNA damage sites |
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ubiquitin-dependent DNA-damage signaling cascade involving the E34 ubiquitin ligases RNF8 and RNF168 plays key roles in coordinating cell cycle progression and DNA repair |
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facilitate recruitment of TP53BP1 to sites of DNA damage and the ubiquitination pathway mediated by RNF8 and RNF168 plays an integral part in class switch recombination |
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with others ATM signalling mediator proteins, MDC1, RNF168 and TP53BP1 are also required for heterochromatic DSB repair |
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RNF8 and RNF168 effect the formation of lysine 63-linked polyubiquitin (K63Ub) chains on damaged chromatin, including on histones HIST2H2AC and H2AFX, in an ATM-dependent manner |
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E3 ubiquitin ligases RNF8 and RNF168 may participate in cellular responses upon replication stress |
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promotes cell survival upon replication stress |
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required for timely progression through the CHEK1-dependent G2 checkpoint following replication arrest |
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participate in DNA damage response including ionizing radiation (IR) |
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has functions in S phase and G2/M phase progression and in the checkpoint mechanism |
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CHFR and RNF8 may have overlapping targets and/or functions in mitosis |
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RNF8 and CHFR affect chromatin relaxation and modulate ATM activation and DNA damage response pathways |
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RNF8 and CHFR, function together to activate ATM and maintain genomic stability |
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RNF8 RING-finger domain is essential for TPP1 stability and retention at telomeres |
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RNF8 and RNF168 are essential RING-E3 ubiquitin ligases that catalyze the formation of Lys-63 ubiquitin chains in the DNA damage response |
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RNF8 dimerization is essential for the optimal function of both its forkhead-associated and RING domains |
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tripartite regulation of homologous recombination by RNF8, BRCA1, and TP53BP1 |
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histone ubiquitination during the DSB pathway is initiated by RNF168 on HIST2H2AC and H2AFX, whereas RNF8 is inactive toward them |
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RNF8-mediated ubiquitination of NBN contributes to the efficient and stable binding of NBN to DNA double-strand break (DSB) and is important for homologous recombination-mediated DSB repair |
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is involved in targeting of the POLD4 for degradation in response to DNA damage |
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RNF8 and RNF168 operate in different modes with their cognate E2 UBE2N at DSBs |
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RNF8 is less abundant than RNF168, and RNF8 is a rate-limiting determinant of focal repair complex assembly |
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RAD18 and RNF8 operate in the same pathway in the promotion of homologous recombination (HR) |
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RNF8 is required for DNA damage repair in neurons |
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RNF8 induces EMT (epithelial-mesenchymal transition) in the breast cancer cells and promotes breast cancer metastasis |
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RNF8 was shown to monoubiquitinate histones H2A and H2B |
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RNF8 and UBE2N are components of a novel cytoplasmic ubiquitin-signaling network that suppresses synapse formation in the brain |
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RNF8 and SCML2 cooperate to regulate ubiquitination during meiosis, an early step to establish active histone modifications for subsequent gene activation |
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RNF8 plays critical roles in maintaining genomic stability by promoting the repair of DNA double-strand breaks (DSBs) through ubiquitin signaling |
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RNF8 regulated NOTCH1 signaling and cell-fate determination of mammary luminal progenitors |
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RNF8 is a central factor in DNA double strand break (DSB) signal transduction |