Selected-GenAtlas references SOURCE GeneCards NCBI Gene Swiss-Prot Ensembl
HGNC UniGene Nucleotide OMIM UCSC
Home Page
FLASH GENE
Symbol CKAP4 contributors: mct/npt/pgu - updated : 11-10-2023
HGNC name cytoskeleton-associated protein 4
HGNC id 16991
RNA
TRANSCRIPTS type messenger
identificationnb exonstypebpproduct
ProteinkDaAAspecific expressionYearPubmed
2 - 3036 63 602 - 2001 11402071
EXPRESSION
Type ubiquitous
   expressed in (based on citations)
organ(s)
SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
Cardiovascularheart   highly
Digestiveliver   highly
Reproductivefemale systemovary  highly
Skin/Tegumentskin   highly
tissue
SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
Muscularsmoothvessel  
cells
SystemCellPubmedSpeciesStageRna symbol
Cardiovascularendothelial cell
cell lineage
cell lines
fluid/secretion
at STAGE
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • a N terminal cytoplasmic domain
  • a single transmembrane domain
  • a rodlike tail sequence highly repetitive,with an heptad repeats forming an helical coiled coil structure involved in subcellular localization
  • conjugated PhosphoP
    mono polymer homomer , oligo
    HOMOLOGY
    Homologene
    FAMILY
  • VDP/USO1/YBl047C family
  • CATEGORY structural protein
    SUBCELLULAR LOCALIZATION     plasma membrane
        intracellular
    intracellular,cytoplasm,organelle,lumen
    intracellular,cytoplasm,organelle,membrane
    intracellular,cytoplasm,organelle,endoplasmic reticulum
    intracellular,cytoplasm,organelle,Golgi
    intracellular,nucleus
    intracellular,nuclear envelope
    text
  • type II transmembrane protein
  • CKAP4 palmitoylation by DHHC2 is required for its trafficking from the ER to the plasma membrane and for its nuclear localization)
  • exclusively localized in sheet-like ER membranes, typical structures of the rough ER, and plays a pivotal role in the static interaction with MTs
  • basic FUNCTION
  • involved in linking the ER to microtubules, and required for protein transport from the ER to the golgi complex
  • may contribute to the functional regulation of the plasminogen activation system in the vessel wall
  • important epithelial cell receptor for APF, Antiproliferative factor (APF) is a sialoglycopeptide that is secreted by bladder cells from interstitial cystitis patients, a potent inhibitor of both normal bladder epithelial and bladder carcinoma cell proliferation
  • CKAP4-mediated stable anchoring of the ER to microtubules is required to maintain the spatial distribution of the ER during interphase and this interaction is abolished by phosphorylation of CKAP4 during mitosis
  • role of CKAP4 as an SFTPA1 receptor protein localized on the surface of lung type II cells
  • role for TP73 and CKAP4 in supporting cellular proliferation through the transcriptional activation of the genes involved in G(1)-S and G(2)-M progression
  • reversibly palmitoylated and phosphorylated protein that links the ER to the cytoskeleton
  • is a gentamicin-binding protein that is involved in drug-induced cytotoxicity
  • RTN and CKAP4 therefore regulate lumenal ER nanodomain heterogeneity, interaction with ER-resident proteins, and dynamics in peripheral ER tubules
  • CKAP4 and the ER luminal protein CALU collaboratively maintain ER sheet morphology
  • acts as an ER luminal bridge
  • during nutrient starvation, cells modulate CLIMP63 protein levels and p180-microtubule binding to bidirectionally move endoplasmic reticulum and lysosomes for proper autophagic responses
  • both RTN4 and CKAP4 ( regulate the recruitment of BAX to ER and mitochondrial membranes to enable cytochrome c release and apoptosis
  • CKAP4 acylation-deacylation controls the abundance and fenestration of ER sheets
  • CELLULAR PROCESS cell organization/biogenesis
    cell communication
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • major substrate of the palmitoyl acyltransferase, DHHC2
  • CKAP4 is required for stabilizing DICER1 protein and for optimal regulation of a reporter gene coupled to the 3prime untranslated region of HMGA2 mRNA in human cells
  • VIMP can interact with CKAP4 and SNX5, it does not interact with MT-binding ER proteins (such as REEP1) that shape the tubular smooth ER
  • CKAP4 is the partner of triadin, and is responsible for the association of triads and microtubules
  • association of TRDN and CKAP4 could be involved in the shaping of SR terminal cisternae and in the guidance of microtubules close to the triads
  • CALU specifically interacts with CKAP4 and antagonizes CKAP4 in terms of both ER sheet distribution and luminal width
  • cell & other
  • microtubules
  • REGULATION
    activated by olomoucine, a CDK inhibitor (strongly up-regulates CKAP4 in a dose- and time-dependent manner solely in senescent cells)
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS