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Symbol AMOT contributors: mct/pgu - updated : 12-04-2016
HGNC name angiomotin
HGNC id 17810
TRANSCRIPTS type messenger
  • not completely determined alternatively spliced transcript variants encoding different isoforms have been described (PMID: 16043488)
  • identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    12 - 6514 72 675 expressed during the migratory phase of retinal angiogenesis 2009 19565639
  • variant 1 - p80
  • promotes angiogenesis, in part, by conferring a hypermigratory phenotype to endothelial cells
  • AMOTL1 associates with this p80-angiomotin-containing complex via its coiled-coil domain
  • is a novel component of the CDH11 complex and Amot-p80 is critical for CDH11-mediated cell migration
  • 11 - 6945 130 1084 expressed during the period of blood vessel stabilization and maturation in retinal angiogenesis 2013 23564455
  • be coprecipitated with MAGI-1b
  • variant 2 - p130
  • N-terminal domain of p130 serves as a targeting domain responsible for localization of p130-AMOT to actin and tight junctions
  • ubiquitinated at lysine 481 by ITCH, resulting in increased AMOT130 stability
  • significant consequence of AMOT130 binding to ITCH is the induction of its ligase activity for self and substrate ubiquitination
  • recruits the E3 protein-ubiquitin ligase atrophin-1 interacting protein 4 (ITCH), that binds and ubiquitinates AMOT130, resulting in a reduction in AMOT130 residence at actin fibers and a significant enhancement of the stability of AMOT130 protein
  • phosphorylation by LATS1/2 is found to be a key feature that enables it to inhibit YAP1-dependent signaling and cell growth
    Type ubiquitous
       expressed in (based on citations)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Cardiovascularheart   highly
     vesselcapillary  highly Homo sapiens
    Digestivemouthtongue  predominantly
    Endocrineneuroendocrinepituitary  moderately
    Lymphoid/Immunespleen   highly
    Nervousbrainbasal nuclei   
     spinal cord    
    Reproductivefemale systemplacenta  moderately
    Urinarykidney   moderately
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Muscular   moderately
    SystemCellPubmedSpeciesStageRna symbol
    Cardiovascularendothelial cell Homo sapiens
    cell lineage
    cell lines
    at STAGE
    physiological period pregnancy
    Text placenta (large vessels)
  • a PPXY motif in the N terminus of AMOT necessary for its interactions , and N-terminal regions contain a conserved HXRXXS consensus site for LATS1/2-mediated phosphorylation
  • a coiled-coil domain
  • a lipid binding domain that bind with high affinity to membranes containing monophosphorylated phosphatidylinositols and cholesterol
  • a consensus motif for binding to PDZ domain at the C terminus, C-terminal PDZ-binding motifs, required for the interactions with AMOTL1, AMOTL2, MPDZ, and INADL
  • angiomotin family, Amot/JEAP family
  • CATEGORY regulatory
    SUBCELLULAR LOCALIZATION     plasma membrane,junction,tight
  • located to the leading edge of migrating endothelial cells
  • actin filament
  • lamellipodium
  • apparent at TJs as well as apical membranes, and mostly co-localized with MPDZ
  • targeted along with associated apical polarity proteins to the endocytic recycling compartment via its membrane binding domain
  • basic FUNCTION
  • mediating angiostatin inhibition of migration and tube formation of endothelial cells
  • stimulating cell motility
  • playing a role in the assembly of endothelial cell-cell junctions
  • maintaining tight junctions integrity by the coordinate regulation of Cdc42 and by linking specific components of the TJ to intracellular protein trafficking, when binding to ARHGAP17
  • important role in growth factor-induced migration of endothelial cells
  • AMOT appears to control the polarity of vascular tip cells whereas AMOTL1 mainly affects the stability of cell-cell junctions of the stalk cells
  • associates with cell junctions and binds apical polarity proteins, which underlie its ability to control cell shape and migration
  • may coordinate the dysregulation of cell polarity with the induction of neoplastic growth in mammary cells
  • functions downstream of Merlin and upstream of ARHGAP17, a small GTPase Activating Protein, as a positive regulator of RAC1
  • activate LATS2 through a novel conserved domain that binds and activates LATS2
  • membrane-associated protein that is expressed in ECs (endothelial cells) and controls migration, TJ (tight junction) formation, cell polarity and angiogenesis
  • AMOT, AMOTL1 and AMOTL2, play critical roles in the Hippo pathway by regulating the subcellular localization of the co-activators YAP1 and TAZ
  • is crucial for the maintenance of nuclear YAP1 to promote renal epithelial and Renal cell carcinoma (RCC) proliferation
  • multifunctional protein involved in endothelial cell migration and tube formation and angiogenesis
  • MAMDC4 and AMOT have a greater association in subconfluent cells compared with confluent cells, and this association is regulated at the endosomal membrane
  • EDIL3, AMOT and ECM1 are involved in the excessive angiogenesis and vasodilation observed in psoriasis
  • CELLULAR PROCESS cell organization/biogenesis
    cell communication
  • actin cytoskeleton organization and biogenesis
  • positive regulation of angiogenesis
  • positive regulation of blood vessel endothelial cell migration
  • tankyrase-RNF146-AMOT axis is an upstream pathway regulating YAP1
  • Hippo pathway negatively regulates the actin-binding activity of AMOT family members through direct phosphorylation
  • a component
  • forms a ternary complex together with INADL (or its paralogue MPDZ) and PLEKHG5, involved in directional migration of capillaries in the embryo
    small molecule
  • angiostatin
  • ARHGAP17 via its coiled-coil domain
  • interacting with MPDZ and INADL (interaction not necessarily responsible for their proper subcellular distribution
  • directly binds the scaffolding proteins INADL and MPDZ and redistributes them and their binding partners from the plasma membrane to endosomes
  • AMOT, AMOTL1 and AMOTL2, are YAP1-associated proteins
  • AMOT and AMOTL1 interact with YAP1 via the first WW domain of YAP1
  • interacting with YAP1 and TAZ (YAP1 and TAZ inhibition by AMOT-mediated tight junction localization)
  • functions downstream of NF2 and upstream of ARHGAP17, a small GTPase Activating Protein, as a positive regulator of RAC1
  • MCAM binds to angiomotin to stimulate a proangiogenic response
  • ITCH bind and ubiquitinate AMOT at residue Lys-481
  • promotes the ubiquitination of YAP1 by ITCH and prevents ITCH from binding to large tumor suppressor 1
  • acts as a YAP1 cofactor, preventing YAP1 phosphorylation and augmenting its activity toward a specific set of genes that facilitate tumorigenesis
  • AMOT is a direct substrate of LATS1/LATS2 mediating functions of the Hippo pathway in endothelial cell migration and angiogenesis
  • LATS1, LATS2 can synergize with F-actin perturbations by phosphorylating free AMOT130 to keep it from associating with F-actin
  • MAMDC4 interacts with both AMOT and occludin and preferentially associates with occludin in confluent cells but with AMOT family members in subconfluent cells
  • MAMDC4 competes with YAP1 for binding to AMOT proteins in subconfluent cells
  • AMOT can interact with YAP1 to either stimulate or inhibit YAP1 activity, playing a potential role in cell proliferation
  • cell & other
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    tumoral     --over  
    in breast cancer tissues and was important in the promotion of breast cancer cell proliferation and invasion
    Variant & Polymorphism
    Candidate gene
    Therapy target
    using angiomotin antibodies for specifically targeting endothelial migration in angiogenesis-dependent diseases