motifs/domains
| N-terminal GRASP domain (amino acids 1-201) with two PDZ domains necessary and sufficient for dimerization and trans-oligomerization but not mitotically regulated (PDZ2 targets the molecule to cis Golgi membranes by binding a PDZ ligand present at the C terminus of cis-localized GOLGA2, whereas PDZ1 likely binds a similarly targeted GORASP1 molecule on an adjacent membrane) |
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two PDZ-like domains, responsible for oligomerization and Golgi membrane stacking, followed by a variable C-terminal region |
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and a zinc finger-like structure is observed in the linker region between the PDZ domains |
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presence of a PLK1 phosphorylation site, Ser189, next to the PDZ ligand sequence suggests several possible inhibitory mechanisms |
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C-terminal serine/proline-rich domain (amino acids 202-446) cannot dimerize nor can it link adjacent surfaces, but confer mitotic regulation on the GRASP domain through multiple sites phosphorylated by the mitotic kinases |
SUBCELLULAR LOCALIZATION
| intracellular
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| intracellular,cytoplasm,organelle,membrane
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| intracellular,cytoplasm,organelle,Golgi
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| intracellular,cytoplasm,cytosolic
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text
| tightly bound to Golgi membrane |
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localize to the cis and medial-trans cisternae |
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localized mainly on the cis-side of the Golgi (23940043) |
basic FUNCTION
| may function as a signal integrator controlling the cell growth |
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involved in establishing the stacked structure of the Golgi apparatus |
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having a role in the regulation of spindle dynamics rather than a direct role in the stacking of Golgi cisternae |
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implicated in the stacking of Golgi cisternae and the regulation of Golgi disassembly/reassembly during mitosis |
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required for Golgi fragmentation during mitosis and mitotic progression in both yeast and mammalian cells |
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required for unconventional protein secretion during development |
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stack mammalian Golgi cisternae via a common mechanism |
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Golgi matrix proteins having recognized roles in maintaining the architecture of the Golgi complex, in mitotic progression and in unconventional protein secretion |
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having a role in the transport of selected cargo along the conventional secretory pathway |
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with GOLGB1, and GOLGA2, facilitates vesicle fusion to |
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the Golgi membrane as a vesicle "tethering factor" |
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GORASP1, GORASP2 are required for formation of the Golgi ribbon, a structure which is fragmented in mitosis owing to the phosphorylation of a number of serine and threonine residues situated in its C-terminus |
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also seems to be the key target of signalling events leading to re-orientation of the Golgi during cell migration and its breakdown during apoptosis |
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GORASP1, GORASP2 are negative regulators of exocytic transport and this slowdown helps to ensure more complete protein glycosylation in the Golgi stack and proper sorting at the trans-Golgi network |
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direct role in Golgi stacking |
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GORASP1, GORASP2 play a direct and cisternae-specific role in linking ministacks into a continuous membrane network |
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GORASP1, GORASP2 specifically link analogous cisternae to ensure Golgi compartmentalization and proper processing |
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implicated in both Golgi stacking and ribbon linking by forming trans-oligomers |