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FLASH GENE
Symbol DBF4 contributors: mct/npt - updated : 04-01-2012
HGNC name DBF4 homolog (S. cerevisiae)
HGNC id 17364
DNA
TYPE functioning gene
SPECIAL FEATURE arranged in tandem
STRUCTURE 33.31 kb     12 Exon(s)
regulatory sequence Promoter
Binding site   transcription factor
text structure
  • a putative MluI Cell-cycle Box (MCB), playing an essential role in the activation of a core promoter and a Sp1 element
  • two initiation zones, two origin recognition complex (ORC) binding sites and two DNase I-hypersensitive regions leading to 'asymmetric bidirectional replication'
  • MAPPING cloned Y linked N status provisional
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    12 - 3923 - 674 - Romero (2008)
    EXPRESSION
    Type ubiquitous
    constitutive of
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Lymphoid/Immunelymph node   highly
    Reproductivemale systemtestis  highly
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    cell cycle     cell cycle, interphase, S, checkpoint, G1S
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a short N-terminal region that targets CDC7-DBF4 kinase to CDC5
  • putative site for phosphorylation by CDK
  • PEST protease cleavage sites, nuclear localization signals (NLS)
  • a short-looped zinc finger-like domain
  • HOMOLOGY
    interspecies homolog to yeast S.cerevisiae DBF4 kinase
    Homologene
    FAMILY
    CATEGORY enzyme , regulatory
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,nucleus
    basic FUNCTION
  • essential for the G1/S transition and initiation of DNA replication to Saccharomyces
  • involved in proliferation, and aggressiveness of prolactin pituitary tumors (Wierinckx 2007)
  • CDC7-DBF4 is a conserved protein kinase required for the initiation of DNA replication
  • CDC7-DBF4 prevents inappropriate exit from mitosis by inhibiting Polo kinase and functions in the spindle position checkpoint
  • CELLULAR PROCESS cell cycle, checkpoint
    nucleotide, replication
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • component of a complex with CDC7 capable of phosphorylating MCM2 protein
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • binding to CDC7 (regulatory partner of the CDC7 serine/threonine protein kinase, which is essential for the activation of individual ori sites throughout S phase in eukaryotic cells)
  • CDC7/DBF4 heterodimer is a novel interactor of PSIP1 (
  • CDC7-DBF4 interaction with CDC5
  • bipartite interaction between CDC7 and DBF4 subunits facilitates ATP binding and substrate recognition by the CDC7 kinase
  • cell & other
    REGULATION
    Other regulated by by transcription and protein degradation.
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS