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FLASH GENE

Symbol

WASF1

contributors: mct - updated : 05-09-2019

HGNC name	WAS protein family, member 1
HGNC id	12732

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

DNA

TYPE	functioning gene
STRUCTURE	80.19 kb     11 Exon(s)

10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

N

status

provisional

Map	cen - D6S434 - D6S283 - D6S1692 - D6S301 - D6S447 - PREP - D6S278 - WASF1 - RPL3 - D6S1635 - D6S1594 - FYN - D6S302 - qter
Authors	Gene Map

RNA

TRANSCRIPTS	type	messenger

text	all transcripts encode the same protein

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_001024934 10 - 3132 NP_001020105 - 559 - 2005 16275905 NM_003931 11 - 3230 NP_003922 - 559 - 2005 16275905 NM_001024936 9 - 2987 BC044591 - 559 - 2005 16275905 NM_001024935 10 - 3085 NP_001020106 - 559 - 2005 16275905

EXPRESSION

Type	ubiquitous

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol blood / hematopoietic thymus         Digestive intestine large intestine colon       intestine small intestine       Endocrine pancreas         Nervous brain       highly Reproductive female system ovary         male system testis     lowly Urinary kidney

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Epithelial secretory glandular endocrine   Epithelial secretory glandular exocrine   Lymphoid         Nervous peripherous

cells

System Cell Pubmed Species Stage Rna symbol Nervous neuron Reproductive spermatid Reproductive spermatocyte

cell lineage

cell lines

fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	a N terminal leucine zipper motif and a highly basic region
	a C terminal VCA region (verproline homology, cofilin homology and acidic region)
	a WH2 domain, playing a role in filament nucleation by Arp2/3

conjugated

PhosphoP

HOMOLOGY

interspecies	homolog to rattus Wasf1 (97.5 pc)
	homolog to murine Wasf1 (98.4 pc)

Homologene

FAMILY

WAS protein family

CATEGORY

regulatory , structural protein

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,cytoplasm,organelle,mitochondria,outer
	intracellular,cytoplasm,organelle,membrane
	intracellular,cytoplasm,organelle,Golgi
	intracellular,cytoplasm,cytoskeleton,microfilament
text	present in dendrites, dendritic spines, and axonal growth cones
	localizes to the outer mitochondrial membrane, where it has been shown to play a role in the trafficking of mitochondria to the dendritic spine

basic FUNCTION	regulator of actin reorganization downstream of RAC
	required for membrane ruffling
	a possible involvement in the pathogenesis of neuroblastoma
	thought to be required for lamellipodium and ruffle formation in cells
	playing a role with its associated proteins in the mid-piece of maturing sperm
	required for activity-dependent mitochondrial trafficking to dendritic spines and filopodia
	role for WASF1 as a critical modulator of the innate immune response to severe bacterial infections
	WASF1 is a maternal reprogramming factor that also has a necessary role in gene activation in development
	has unique activities independent of Arp2/3 complex that can govern both the growth rates and architectures of actin filament networks
	playing a role in mitochondrial movement into dendritic protrusions
	WAS and WASF1 may both be required to build pseudopods
	plays a critical role in binding actin to initiate actin polymerization

CELLULAR PROCESS

cell organization/biogenesis

PHYSIOLOGICAL PROCESS

text	cell shape and cell-cycle control

PATHWAY

metabolism

signaling

a component

associating with the Arp2/3 complex, a nucleating core constituent for actin polymerization in vitro

INTERACTION

DNA

RNA

small molecule

protein	with arp2/3 (activated by the VCA region)
	RAPH1 directly binds active RAC1, which regulates a direct interaction between RAPH1 and the WASF1 complex via ABI1
	WASF1 interacts and colocalizes with APP in the Golgi apparatus
	is a critical pro-autophagic protein capable of enhancing cell survival and regulating chemoresistance in leukemia cells potentially through the BECN1/BCL2 and BECN1/PI3K-&
	8546; complex-dependent pathways
	NCKAP1 is a cell invasion promoter by binding to WASF1, but also a tumor suppress gene that modulates the cell cycle of hepatocellular carcinoma (HCC) cell lines by targeting RB1/TP53 regulation

cell & other

REGULATION

inhibited by

phosphorylated at multiple sites by cyclin-dependent kinase 5 (Cdk5)

ASSOCIATED DISORDERS

corresponding disease(s)

MRD59

Susceptibility

Variant & Polymorphism

Candidate gene
Marker	independent prognostic factor for epithelial ovarian cancer (EOC), which suggests that it is a novel and crucial predictor for EOC metastasis
Therapy target

ANIMAL & CELL MODELS