protein
| CWC22 and DHX16 bind to the spliceosome independently of each other (CWC22 is required for the productive function of DHX16 in the first catalytic step, but in the absence of CWC22, DHX16 binds to the spliceosome and dissociates upon ATP hydrolysis in a nonproductive manner) |
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CWC22 is a new EIF4A3 partner (duality of CWC22 function that hinders EIF4A3 from nonspecifically binding RNA and escorts it to the splicing machinery to promote exon-junction complex (EJC) assembly on mature mRNAs) |
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CWC22 directly interacts with the core exon junction complex (EJC) component EIF4A3 |
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CWC22 represents likely an integral component of the spliceosome and orchestrates pre-mRNA splicing and EIF4A3 binding to achieve global assembly of exon junction complexes |
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SLU7 adopts an extended conformation, binds PRPF8 and CWC22, and is poised for selection of the 3'-splice site |
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phosphorylation of EIF4A at threonine 163 (T163), promotes association of EIF4A3 with CWC22, which guides EIF4A3 to an active spliceosome |
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splicing factor CWC22 is the major CWC27 partner |
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CWC27 functions as a splicing factor, interacting with CWC22 to form a landing platform for EIF4A3, a core component of the exon junction complex |