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FLASH GENE
Symbol CWC22 contributors: mct - updated : 06-12-2022
HGNC name CWC22 spliceosome-associated protein homolog (S. cerevisiae)
HGNC id 29322
DNA
TYPE functioning gene
STRUCTURE 62.18 kb     20 Exon(s)
MAPPING cloned Y linked N status provisional
RNA
TRANSCRIPTS type messenger
identificationnb exonstypebpproduct
ProteinkDaAAspecific expressionYearPubmed
20 - 3279 - 908 - 2020 32329775
19 - 3007 - 867 - 2020 32329775
18 - 2807 - 606 - 2020 32329775
20 - 3130 - 908 - 2020 32329775
20 - 3269 - 908 - 2020 32329775
EXPRESSION
Type
   expressed in (based on citations)
organ(s)
cell lineage
cell lines
fluid/secretion
at STAGE
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • an MIF4G-like domain in the N-terminal region
  • a MA-3 domain in the middle region
  • HOMOLOGY
    Homologene
    FAMILY
    CATEGORY RNA associated
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,cytosolic
    intracellular,nucleus,nucleoplasm,nuclear bodies,nuclear speckles
    text
  • associated with the spliceosome prior to catalytic steps and remains associated throughout the reaction
  • basic FUNCTION
  • required for pre-mRNA splicing but is neither tightly associated with the nineteen complex [NTC] nor required for spliceosome activation
  • might be associated with the spliceosome in a step after the binding of the NTC but prior to the first catalytic reaction
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • CWC22 and DHX16 bind to the spliceosome independently of each other (CWC22 is required for the productive function of DHX16 in the first catalytic step, but in the absence of CWC22, DHX16 binds to the spliceosome and dissociates upon ATP hydrolysis in a nonproductive manner)
  • CWC22 is a new EIF4A3 partner (duality of CWC22 function that hinders EIF4A3 from nonspecifically binding RNA and escorts it to the splicing machinery to promote exon-junction complex (EJC) assembly on mature mRNAs)
  • CWC22 directly interacts with the core exon junction complex (EJC) component EIF4A3
  • CWC22 represents likely an integral component of the spliceosome and orchestrates pre-mRNA splicing and EIF4A3 binding to achieve global assembly of exon junction complexes
  • SLU7 adopts an extended conformation, binds PRPF8 and CWC22, and is poised for selection of the 3'-splice site
  • phosphorylation of EIF4A at threonine 163 (T163), promotes association of EIF4A3 with CWC22, which guides EIF4A3 to an active spliceosome
  • splicing factor CWC22 is the major CWC27 partner
  • CWC27 functions as a splicing factor, interacting with CWC22 to form a landing platform for EIF4A3, a core component of the exon junction complex
  • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    constitutional     --other  
    aberrantly upregulated in diabetic dorsal root ganglia (DRG), and impaired neuronal function
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    ANIMAL & CELL MODELS