motifs/domains
| N terminus, with a leucine zipper domain (AA 130–151) that could be involved in mediating protein-protein or protein-DNA interactions |
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three nuclear localization signals |
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three ATM/ATR phosphorylation motifs |
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a monoubiquitination site |
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ten S/TQ motifs, with multiple phosphorylation sites that are functionally important for inducing monoubiquitination of FANCD2, a crucial event in the Fanconi anemia pathway and interstrand cross-links repair |
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an EDGE motif that is critical for the ability of FANCI to properly monoubiquitinate FANCD2 and promote DNA crosslink resistance |
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C-terminus may be involved in the regulation of DNA binding activity, but dispensable for direct DNA binding |
basic FUNCTION
| involved in the replication of mitochondrial DNA |
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DNA damage-responsive protein that functions similarly to FANCD2 |
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having a monoubiquitination-independent role in DNA repair, such as in the regulation of FANCD2 protein stability |
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required to prevent accumulation of replication-associated DNA double-strand breaks |
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required for maintenance of chromosomal stability |
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involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links |
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participating in S phase and G2 phase checkpoint activation upon DNA damage |
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promoting FANCD2 ubiquitination and recruitment to DNA repair sites |
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ATR-dependent phosphorylation of FANCI is a molecular switch for the Fanconi anemia pathway |
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monoubiquitination of FANCI is largely dispensable for the DNA repair function of the Fanconi anemia pathway |
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ubiquitylated FANCI-FANCD2 controls interstrand cross-links repair in S phase |
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upon the occurrence of DNA damage, FANCI becomes monoubiquitinated on Lys-523 and relocalizes to chromatin, where it functions with monoubiquitinated FANCD2 to facilitate DNA repair |
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FANCD2 and FANCI function together in the Fanconi anemia network of deoxyribonucleic acid (DNA) crosslink repair |