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FLASH GENE
Symbol PRPF19 contributors: mct - updated : 24-05-2023
HGNC name PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
HGNC id 17896
DNA
TYPE functioning gene
STRUCTURE 16.04 kb     16 Exon(s)
MAPPING cloned Y linked N status provisional
RNA
TRANSCRIPTS type messenger
identificationnb exonstypebpproduct
ProteinkDaAAspecific expressionYearPubmed
16 - 2342 - 504 - 2010 2017681
EXPRESSION
Type ubiquitous
   expressed in (based on citations)
organ(s)
SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
Digestivestomach   highly
Reproductivefemale systemuteruscervix highly
 male systemprostate  highly
tissue
SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
Connectivebone  highly
cell lineage
cell lines
fluid/secretion
at STAGE
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • a domain of AAs 167-250 is necessary for the recruitment of PRPF19 to lipid droplets and a domain of AAs 1-166 is necessary for the recruitment of PRPF19 to a nucleus
  • C-terminal WD40 repeat domain, involved in variety of processes in eukaryotic cells
  • secondary structure WD40 domain with a canonical seven-bladed beta-propeller fold, consisting of seven WD40 repeats
    HOMOLOGY
    interspecies ortholog to yeast Prp19
    Homologene
    FAMILY
    CATEGORY enzyme
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,cytosolic
    intracellular,cytoplasm,cytoskeleton,microtubule,mitotic spindle
    intracellular,nucleus,nucleoplasm,nuclear bodies,nuclear speckles
    text
  • found in the nucleus, cytosol or lipid droplets depending on the cell type
  • basic FUNCTION
  • playing a role in pre-mRNA splicing, but also having a direct role in DNA damage processing
  • extending the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells
  • nuclear matrix protein involved in pre-mRNA splicing, ubiquitylation, and DNA repair
  • involved in pre-mRNA splicing, having an essential role for spliceosome assembly and spliceosome stability
  • is necessary to bring SETMAR to the DSB sites for its function(s) in DNA repair
  • once it forms a complex with SETMAR, negatively regulates its TIR binding activity, which is perhaps necessary for SETMAR localization at non-TIR sites such as double-strand breaks
  • spliceosomal PRPF19 complex modifies PRPF3, a component of the USP4, with nonproteolytic K63-linked ubiquitin chains
  • multi-functional protein that plays a role in splicing, ubiquitination, and cell growth
  • both U2AF2 and PRPF19 are required for RNA polymerase II C-terminal domain-dependent splicing activation
  • PRPF19 splicing complex is potentially a novel transcription elongation factor
  • involved in cellular life-span regulation and proteasomal breakdown
  • PRPF19 participated in mitotic progression in hepatocellular carcinoma (HCC)
  • PRPF19 plays an important role in the induction of TP53-dependent cellular senescence 7)
  • PRPF19 promotes cell viability and slows down the aging process in human skin
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • PRPF19 and its related proteins are major components of the spliceosome catalytic core RNP
  • PRPF19/CDC5L complex contains four copies of PRPF19
  • important component in human spliceosome machinery
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • interact with WRN through a direct physical association between WRN and CDC5L
  • SETMAR binding partner that mediates SETMAR binding to non-TIR DNA such as DNA damage sites
  • is involved in DNA repair and splicing, associates with the proteasome by directly binding to the beta7 subunit of the 20 S proteasome
  • CTNNBL1 associates with the PRPF19 complex of the spliceosome, interacting with its CDC5L component
  • BCAS2 directly interacts with each component of the PRPF19/CDC5L complex core
  • interacts with PHD3 via its C-terminal WD40 region, and the interaction is enhanced under hypoxic conditions through the utilization of the N-terminal coiled-coil domain
  • CTNNBL1 associated with PRPF19-containing RNA-splicing complexes as well as with the antibody-diversifying enzyme AICDA
  • SNW1-associated factors DHX8 and PRPF19 are also selectively required for CDKN1A expression under stress
  • EXOC7 interacts directly with PRPF19 and shuttles to the nucleus, where it associates with the spliceosome
  • DHX15 and PRPF19, two spliceosome components, are novel RBM5-interacting partner
  • CCDC94 and the PRPF9 complex protect cells from IR-induced apoptosis by repressing the expression of TP53 mRNA
  • PRPF19 complex is the first spliceosome subcomplex that directly contributes to mitosis in vertebrates independently of its function in interphase
  • BCAS2 and PRPF19 subunits of the PSO4 complex directly interact and colocalize with replication protein A (RPA1)
  • PRPF19 interacts with CDC5L directly
  • PRPF19 downregulation inhibits MDM4-mediated TP53 inactivation, resulting in induction of cellular senescence 7)
  • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    constitutional     --low  
    in various cell types during replicative senescence
    tumoral     --over  
    in various immortalized cell lines, as well as in breast tumors, with good survival
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    SystemTypeDisorderPubmed
    cancerdigestiveliver
    could be a promising therapeutic target of HCC
    ANIMAL & CELL MODELS