protein
| binding and associating with NCF4 for phox expression and granulocytic differentiation |
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regulating expression of crucial myeloid genes such as CSF1, CSF2, CSF3 |
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interazcting with GATA1 (Ets domain of SPI1 and the GATA1 C-terminal zinc finger (CF) form a low affinity interaction in which specific regions of each protein are implicated) |
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interacting with NFATC1 (SPI1 confers specificity to the NFATC1 response to macrophage lineage cells) |
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interacting with TRIM33 (regulation of adult hematopoiesis through TRIM33-mediated transcriptional repression of TAL1 and SPI1 target genes) |
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SPI1 is a critical factor for the expression of CD80 and CD86 |
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RUNX1 cooperates with SPI1 to activate myeloid differentiation genes, such as macrophage and granulocyte macrophage colony-stimulating factor receptors (MCSFR and GMCSFR) |
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MT1A is a direct target gene negatively regulated by SPI1 |
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SPI1 and GATA1 transactivate FCER1A |
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is a critical regulator of CITED2, as SPI1 repressed CITED2 expression in a DNMT3A/B-dependent manner in normal CD34+ cells |
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TCF3 antagonism of SPI1 activity contributes likely to its ability to commit multipotential hematopoietic progenitors to the lymphoid lineages |
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critical role of SPI1 in the regulation of TLR1, 2, 4 and of CD14 |
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mechanistically, METTL14 exerts its oncogenic role by regulating its mRNA targets (e.g., MYB and MYC) through N6-methyladenosine (m6A)modification, while the protein itself is negatively regulated by SPI1 |