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FLASH GENE
Symbol CLIP1 contributors: shn - updated : 26-09-2010
HGNC name CAP-GLY domain containing linker protein 1
HGNC id 10461
DNA
TYPE functioning gene
SPECIAL FEATURE head to head
STRUCTURE 151.14 kb     25 Exon(s)
MAPPING cloned Y linked N status confirmed
Map cen - D12S2073 - D12S1349 - CLIP1 - D12S1603 - D12S1612 - qter
RNA
TRANSCRIPTS type messenger
identificationnb exonstypebpproduct
ProteinkDaAAspecific expressionYearPubmed
24 - 5793 - 1392 - 1998 9784600
also called CLIP170 (11) or isoform b
25 - 5898 - 1427 skeletal muscle 1998 9784600
  • also called CLIP170 (11+35) or Isoform a
  • with the 11 and 35 AA inserts in tandem
  • - - - - 972 - 2010 20664522
  • accumulates at kinetochores, consequently, this truncated form might compete with and displace endogenous CLIP1 from kinetochore-binding sites
  • EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Endocrinethyroid   highly
    Nervousspinal cord    
    Reproductivefemale systemplacenta  highly
    Respiratoryrespiratory tractlarynx  highly
    Urinarykidney   moderately
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Epithelialsecretoryglandularexocrine 
    Muscularstriatumskeletal  
    Nervouscentral   
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    cell cycle     cell cycle, interphase
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • two N terminal microtubule binding domains
  • a large coiled-coil alpha helical domain
  • a cysteine-rich tail
  • C-terminal zinc-finger domain is responsible for its interaction with dynactin
  • conjugated PhosphoP
    HOMOLOGY
    interspecies ortholog to Clip1, Mus musculus
    ortholog to Clip1, Rattus norvegicus
    ortholog to CLIP1, Pan troglodytes
    Homologene
    FAMILY
    CATEGORY structural protein
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,organelle,endosome
    intracellular,cytoplasm,cytoskeleton,microtubule
    intracellular,cytoplasm,cytoskeleton,intermed filament
    intracellular,nucleus,nucleoplasm
    intracellular,nucleus,chromatin/chromosome,kinetochore
    text
  • at the onset of mitosis, localizes to kinetochores, but at metaphase, it is no longer detectable at kinetochores
  • binds to microtubules in an N-terminal domain-dependent manner and this interaction is regulated by both conformational changes
  • CLIP1 colocalizes with Polo-like kinase 1 (PLK1) at kinetochores during early mitosis
  • basic FUNCTION
  • associated specifically with the ends of growing microtubules and may act as anti-catastrophe factors
  • plays pivotal roles in orientation of microtubules
  • regulates microtubule dynamics and has been implicated in cargo-microtubule interactions
  • microtubule 'plus end tracking' protein involved in several microtubule-dependent processes in interphase
  • facilitates the formation of kinetochore-microtubule attachments, possibly through direct capture of microtubules at the kinetochore
  • implicated in the formation of kinetochore–microtubule attachments through direct interaction with the dynein/dynactin complex
  • MAPRE1, MAPRE2 and CLIP1 are two well-studied microtubule plus-end-tracking proteins (+TIPs) that target growing microtubule plus ends in the form of comet tails and regulate microtubule dynamics
  • MAPRE2 and CLIP1 cooperatively regulate microtubule dynamic instability at concentrations below which neither protein is effective
  • outer kinetochore protein, having a role in kinetochore-microtubule attachment and chromosome alignment during mitosis
  • acts as a novel recruiter and spatial regulator of PLK1 at kinetochores during early mitosis, promoting K-fiber stability and chromosome alignment for error-free chromosome segregation
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • CLIP1–MAPRE1 complex undergoes multiple cycles of binding and dissociation from growing microtubule ends
  • the most important substrates of AMPK
  • INTERACTION
    DNA
    RNA
    small molecule other,
  • growing ends of microtubules
  • binds tubulin dimers
  • protein
  • dynactin complex and the APC-binding protein EB1
  • IQ motif containing GTPase activating protein 1, IQGAP1
  • FKBP12-rapamycin-associated protein (FRAP, also called mTOR/RAFT)
  • lissencephaly-1 protein, LIS1
  • interacts with dynactin through its C-terminus, and this interaction is critical for the essential function of CLIP1 in the formation of kinetochore–microtubule attachments
  • protein kinase, AMP-activated, alpha 2 catalytic subunit, protein kinase, AMP-activated, alpha 2 catalytic subunit
  • interacting with MTOR (MTOR controls dendritic arbor morphology by enhancing cross talk between dynamic microtubules and actin through CLIP1 and IQGAP1)
  • interaction of SLAIN2 with MAPRE1 and CLIP1
  • cell & other
  • microtubule binding protein
  • intermediate filament associated
  • REGULATION
    Other phosphorylated by PRKAA2
    phosphorylated by CSNK2A1 (CSNK2A1 phosphorylation is involved in its dynactin-mediated kinetochore localization)
    its phosphorylation by PLK1 and CSNK2A1 is essential for the timely formation of microtubule–kinetochore attachments
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    tumoral     --over  
    in the reed-Sternberg cells of Hodgkin's disease
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    SystemTypeDisorderPubmed
    cancerangiogenesis 
    interaction between AMPK and CLIP-170 might be a therapeutic target for treatment of conditions such as cancer, tumour angiogenesis and neointimal hyperplasia
    ANIMAL & CELL MODELS