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FLASH GENE
Symbol ITSN2 contributors: mct - updated : 13-06-2016
HGNC name intersectin 2
HGNC id 6184
Location 2p24.1      Physical location : 24.425.734 - 24.583.397
Synonym name
  • SH3P18-WASP associated protein homolog
  • SH3P18-like WASP associated protein
  • SH3 domain protein 1B
  • Synonym symbol(s) KIAA1256, SWAP, SH3P18, SH3D1B, SWA, ITN2, PRO2015
    DNA
    TYPE small rna
    STRUCTURE 157.66 kb     39 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked N status provisional
    RNA
    TRANSCRIPTS type messenger
    text additional variants have been found but their full length nature has not been determined
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    40 splicing 6112 193.3 1697 - 2001 11690630
  • ITSN2L (variant 1)
  • contains a C-terminal extension encoding a DH, a PH and a C2 domain
  • role of ITSN-2L has been shown in regulating actin remodeling and mitotic spindle orientation
  • 31 - 4597 142 1249 - 2001 11690630
  • ITSN2S1 (variant 2)
  • lacking the extra C domains
  • 39 splicing 6031 190.5 1670 relative increase in adult human brain tissue, may be specifically expressed in neurons hinting to a physiologic role in neuronal cell function 2007 17390067
  • ITNS2S2 (variant 3), or ITSN2C
  • minor variant lacking exon 7
  • EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Cardiovascularheart    
    Digestiveliver    
    Reproductivemale systemprostate   
    Urinarykidney    
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • two EH domains
  • a coiled-coil region
  • five SH3 domains of ITSN2 bound well-established interactors of ITSN1 as well as newly identified ITSNs protein partners
  • a EF-hand calcium-binding domain
  • C-terminal extension encoding a Dbl homology domain (DH), a pleckstrin homology domain (PH) and a C2 domain, suggesting that it could act as a guanine nucleotide exchange factor for Rho-like GTPases, with possible roles of C2 domain in regulating the activity of CDC42
  • HOMOLOGY
    Homologene
    FAMILY
  • SH3P18-WASP associated protein
  • member of a family of proteins involved in clathrin-mediated endocytosis
  • CATEGORY adaptor , transport
    SUBCELLULAR LOCALIZATION extracellular
        intracellular
    intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
    basic FUNCTION
  • regulating the formation of clathrin-coated vesicles
  • functioning in the induction of T cell antigen receptor (TCR)
  • SH3/SH2 adaptor protein
  • ITSN1 and ITSN2 are conserved proteins involved in clathrin-mediated endocytosis
  • guanine nucleotide exchange factor for CDC42, regulating different steps of caveola endocytosis in Endothelial cells by controlling the temporal and spatial actin polymerization and remodeling sub-adjacent to the plasma membrane
  • multimodule scaffolding protein functioning in membrane-associated molecular trafficking and signal transduction pathways
  • ITSN1 and ITSN2, are crucial for the initiation stage of clathrin-mediated endocytosis
  • during evolution of vertebrates ITSN2 acquired a novel protein-interaction interface that allows its specific recognition by the SH2 domains of signaling proteins
  • ITSN1, ITSN2 are scaffold proteins involved in the molecular mechanisms of invadopodia formation
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS endocytosis transport
    PATHWAY
    metabolism
    signaling
    a component
  • belong to a complex containing multimers of ITSN1, ITSN2 and EPS15 and differents partners
  • ITSN1, ITSN2 form homomeric and heteromeric complexes with each other revealing an added level of complexity in the function of these evolutionarily conserved scaffolds
  • INTERACTION
    DNA
    RNA
    small molecule metal binding,
  • Ca2+
  • protein
  • bindind to EPS15
  • binding factor of EPS8, and ITSN2 interacts with EPS8 and stimulates the degradation of EPS8 proteins
  • ITSN2 is a major transducer of ING1a-induced senescence signaling
  • WIPF1 interacts with endocytic adaptor proteins of the intersectin (ITSN) family, ITSN1 and ITSN2
  • WIPF3 acts as a modulator of ITSN1, ITSN2 scaffold proteins association with actin
  • ITSN1 and ITSN2 are podocytic guanine nucleotide exchange factors for CDC42
  • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS