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FLASH GENE

Symbol

EP400

contributors: mct - updated : 01-10-2014

HGNC name	E1A binding protein p400
HGNC id	11958

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

EXPRESSION

Type	ubiquitous

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Endocrine parathyroid       highly Lymphoid/Immune lymph node       highly Nervous brain

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Connective adipose     highly

cell lineage

cell lines

fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	an helicase ATP-binding domain
	an helicase C-terminal domain
	a HSA domain
	a Myb-like domain
	a conserved SWI3-ADA2-N-CoR-TFIIIB (SANT) domain which interact both with histone tails and with other proteins and is further implicated in both positive and negative chromatin-regulatory modifications

HOMOLOGY

Homologene

FAMILY	SNF2/RAD54 helicase family
	SWI2/SNF2 family of chromatin remodeling proteins

CATEGORY

regulatory

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,nucleus,nucleoplasm,nuclear bodies,nuclear speckles

basic FUNCTION	may be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation
	represses CDKN1A expression in unstressed cells, thereby allowing cell cycle progression and DNA damage-induced apoptosis
	inhibits TP53 --> CDKN1A transcription and the development of premature senescence
	SWI2/SNF2-related ATPase that serves as an ATP-dependent chromatin remodeling enzyme
	represses basal CDKN1A gene expression through dual mechanisms that include the direct inhibition of KAT5 enzymatic activity and the previously described ATP-dependent positioning of H2AFZ at the promoter
	through an ATP-dependent chromatin remodeling activity that facilitates H2AFZ deposition, acts to repress specific genes (CDKN1A) in this pathway
	ATP-dependent chromatin-remodeling protein that catalyzes the deposition of histone variant H2A.Z into nucleosomes to regulate gene expression
	plays a key role in cellular proliferation by controlling the expression of cell-cycle-regulatory genes
	is a novel DNA damage response protein and EP400-mediated alterations in nucleosome and chromatin structure promote both chromatin ubiquitination and the accumulation of BRCA1 and TP53BP1 at sites of DNA damage
	EP400 ATPase is required for DNA repair by homologous recombination (HR)
	is important for HR-dependent processes, such as recruitment of RAD51 to DSB (a key component of HR), homology-directed repair, and survival after DNA damage

CELLULAR PROCESS

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

a component	component of the NuA4 histone acetyltransferase complex
	component of the p53-p21(WAF1/CIP1/sid1) pathway, regulating the CDKN1A transcription and senescence induction program

INTERACTION

DNA

RNA

small molecule	nucleotide,
	ATP

protein	interacting with MYC, an essential downstream target of E1A
	interacting with KAT5 (KAT5 and EP400, which belongs to the Tip60 complex, are also required for DNA damage-induced apoptosis)
	interacting with H2AFZ (required for the localization of H2AFZ, and largely colocalizes with H2AFZ at multiple promoters)
	interaction between KAT5 and EP400 involving the SANT domain, which binds to the HAT domain of KAT5 and suppresses HAT activity
	HDAC6 plays a major role in the modulation of KAT5-EP400 function in stem cells

cell & other

REGULATION

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral fusion       EP400-PHF1, was discovered in ossifying fibromyxoid tumor

Susceptibility

Variant & Polymorphism

Candidate gene

Marker

Therapy target

ANIMAL & CELL MODELS