Selected-GenAtlas references SOURCE GeneCards NCBI Gene Swiss-Prot Ensembl
HGNC UniGene Nucleotide OMIM UCSC
Home Page
FLASH GENE
Symbol INO80 contributors: mct/npt/pgu - updated : 31-08-2015
HGNC name INO80 complex homolog 1 (S. cerevisiae)
HGNC id 26956
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • N-terminal domain, dispensable for the nucleosome remodeling activity, is important for INO80 to interact with tubulin and co-localize with the spindle
  • a 7-motif SNF2 helicase domain
  • a SNF domain motif-1
  • a SNF2 domain at a central region of INO80 with a potential structural and possibly functional interaction of both RVB1/2 and INO80 ATPases
  • a DBINO (DNA-binding domain of INO80) domain
  • two nuclear localization signals
    • mono polymer heptamer
    HOMOLOGY
    interspecies homolog to murine Inoc1 (97.0pc)
    Homologene
    FAMILY
  • subfamily of SWI2/SNF2 chromatin remodeling proteins
  • INO80/SWR1 family chromatin remodelers
    • CATEGORY enzyme , regulatory
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,cytoskeleton,microtubule
    intracellular,nucleus
    text
  • detected at the DNA damage sites in all interphase cells
    • basic FUNCTION
  • having DNA-binding activity, may be a DNA helicase
  • important members of the chromatin remodeling complexes that are implicated in epigenetic control of gene expression
  • has roles in transcription, DNA repair and replication
  • playing a direct role in fork elongation, and being an important new player in DNA replication to help safeguard the integrity of the genome
  • has a crucial role in stabilizing a stalled replisome to ensure proper restart of DNA replication
  • has roles in transcriptional regulation, DNA double-strand break (DSB) repair and regulation of the DNA-damage cell-cycle checkpoint response
  • requiered by YY1 to YY1-activated genes, where it functions as an essential coactivator
  • remodeling enzyme recruited to replication origins as cells enter S phase
  • its chromatin-remodeling activity regulates efficient progression of replication forks and has a crucial role in stabilizing a stalled replisome to ensure proper restart of DNA replication
  • required for efficient S-phase progression
  • proteasome with the INO80 chromatin-remodeling complex may cooperate to regulate transcription or DNA repair, processes in which both complexes have been implicated
  • assists DSB (double-strand break) repair by positively regulating the expression of the RAD54B and XRCC3 genes
  • can contribute to DSB repair indirectly via gene expression, suggesting that the mechanistic role of this chromatin remodeller in DSB repair is evolutionarily diversified
  • crucial role of the INO80 complex in DNA damage response
  • critical role in creating DNA accessibility for the nucleotide excision repair (NER) pathway and suggesting that repair of UV lesions and perhaps most bulky adduct lesions requires chromatin reconfiguration
  • INO80 ATP-dependent chromatin remodeling complex is a nucleosome spacing factor
  • regulates the genome-wide distribution of H2AFZ and it promotes the eviction of H2AFZ from promoters during transcriptional induction
  • catalyzes the removal of unacetylated H2AFZ from chromatin as a mechanism to promote genome stability
  • capable of catalyzing both ATP-dependent nucleosome sliding and DNA-dependent ATPase
  • is required for efficient double-strand break repair, mediating 5prime-3prime resection of double-strand break ends
  • can play a direct role in the spindle assembly independent of its chromatin remodeling activity
  • INO80 chromatin remodeling complex functions in transcriptional regulation, DNA repair, and replication
  • role of monomeric actin in INO80 chromatin remodeling
  • plays important roles in transcriptional regulation and DNA replication and repair, and consists of several functional protein subunits, including the critical INO80 ATPase catalytic subunit
  • is structurally and mechanistically distinct from SWI/SNF-type remodelers, and instead of providing a nucleosome-binding pocket, INO80 forms a flexible clamp in histone variant exchange and nucleosome remodeling
  • has a distinct mechanism of interacting with the nucleosome by forming a flexible cradle that could partially embrace the nucleosome
    • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • catalytic subunit of a multisubunit, ATP-dependent chromatin-remodeling complex
  • INO80 chromatin-remodeling complex (preferential localization of multiple subunits of the INO80 complex to telomeres)
  • regulates transcription as well as DNA repair and replication processes
  • INO80 complex also contains three actin-related proteins (ARPs), of which ACTR5 and ACTR8 are specific to the INO80 complex
  • composed of three modules that assemble with three distinct domains of the INO80 ATPase
  • INO80 chromatin remodeling complex, comprising the INO80 and the associated proteins such as RUVBL1, has been implicated in a variety of nuclear processes other than transcription
    • INTERACTION
    DNA binding
    RNA
    small molecule nucleotide,
  • ATP
    • protein
  • recruited by YY1 to YY1-activated genes, where it functions as an essential coactivator
  • interacts with tubulin and co-localizes with the mitotic spindle and is required for spindle formation
  • DDB1 interacts with the INO80 complex providing a mechanistic link between chromatin remodeling activity and the initiating step of nucleotide excision repair
  • binds to the promoters of the RAD54B and XRCC3 genes
  • interacting with ACTR8 (ACTR8 is an important subunit that is required for the recruitment of the mammalian INO80 complex to the DNA damage sites)
  • interactions between the nucleosome histone core and ACTR8 in the INO80 chromatin remodeling complex
  • H2AFZ removal from chromatin is likely the primary function of INO80 and ANP32E in promoting homologous recombination
    • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    constitutional        
    loss of INO80 abolished the assembly of nucleotide excision repair (NER) factors, suggesting that prior chromatin relaxation is important for the NER incision process
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    ANIMAL & CELL MODELS