motifs/domains
| N-terminal 80 AAs domain required for binding to atlastin |
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a putative nuclear localization signal (NLS) the AAA domain chaperone like adenosine triphosphatases (ATPase associated with diverse cellular activities) |
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two leucine zipper domains |
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a coiled-coil dimerization motif |
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a proline/serine/threonine/glycine-rich domain important for microtubule binding of spastin between AAs 227 and 342 (sometimes called linker), and MTBD, microtubule binding domain, AAs 270–328 |
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a three-helix domain from AA 116 to 196 with homology to other enzymes involved in various cellular processes (termed the MIT domain (for “contained within microtubule-interacting and trafficking molecules)  , dispensable for ATPase and severing activities |
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C-terminal AAA+ ATPase domain forms a hexamer around a central pore, AAs 342–599 , essential for microtubule severing, from AA 343 to the C terminus (AA 616) , , and the presence of domain iv is necessary and sufficient for the interaction with microtubules |
SUBCELLULAR LOCALIZATION
| intracellular
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| intracellular,cytoplasm,organelle,endosome
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| intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
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| intracellular,cytoplasm,cytoskeleton,microtubule,mitotic spindle
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| intracellular,nucleus
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| text
| perinuclear distribution |
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co-localization with microtubule of an ATPase-defective spastin |
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onset expression in microtubule-organizing center (tranfection experiments) |
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localize to the endosomal membrane traffic compartment, suggesting that the long axons of the corticospinal tract may be especially vulnerable to endosomal dysfunction |
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centriolar localization |
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localize prominently to the midbody |
| basic FUNCTION
| putative chaperone protein involved in the transport of dopamine-containing membranous vesicles and in membrane-microtubule interactions |
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playing a role in cytoskeletal rearrangements and dynamics |
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the transient association to microtubules is regulated through the ATPase activity of the AAA domain |
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playing a role in membrane traffic and in microtubule dynamics an in maintenance of motor neuron function |
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microtubule-severing proteins SPAST and KATNA1 participate differently in the formation of axonal branches |
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may selectively recognize post-translationally modified tubulins (Glu tubulins) that are part of stable microtubules |
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implicated in cytoskeletal rearrangement and dynamics |
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inhibitor of BMP signalling in neuronal and non-neuronal cells |
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microtubule-severing enzyme, critically important for the development of the nervous system, and also for its maintenance throughout adult life |
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is active in the form of homohexameric rings that dynamically assemble and disassemble |
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microtubule severing ATPase that regulates intracellular and axonal transport of vesicles |
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promotes the formation of microtubule networks that are essential for axon growth and branching which are important for neuronal plasticity |
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hexameric ring AAA ATPase that severs microtubules |
