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FLASH GENE
Symbol RAB35 contributors: mct/npt/pgu - updated : 28-04-2015
HGNC name RAB35, member RAS oncogene family
HGNC id 9774
EXPRESSION
Type ubiquitous
   expressed in (based on citations)
organ(s)
SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
Lymphoid/Immunethymus   highly
Nervousbrain   highly
Reproductivemale systemprostate  highly
cell lineage
cell lines
fluid/secretion
at STAGE
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • four conserved domains important for GTP binding
  • the C-terminus involved in membrane association
  • C-terminal polybasic cluster of the RAB35 hypervariable C-terminal domain (HVD) is essential for plasma membrane (PM) targeting, presumably because of the electrostatic interaction with negatively charged lipids on the PM
    • conjugated LipoP , Other
    HOMOLOGY
    interspecies homolog to Drosophila CG9575
    homolog to C.elegans 4R79.2
    Homologene
    FAMILY
  • small GTPase superfamily
  • Rab family
    • CATEGORY protooncogene , signaling
    SUBCELLULAR LOCALIZATION extracellular
        plasma membrane
        intracellular
    intracellular,cytoplasm,organelle,mitochondria
    intracellular,cytoplasm,organelle,membrane
    intracellular,cytoplasm,organelle,endosome
    intracellular,cytoplasm,cytosolic
    intracellular,nucleus
    text
  • accumulates at cell-cell contacts in a cadherin-dependent manner
  • RAB35 accumulates at ARF6-positive endosomes in response to nerve growth factor (NGF) stimulation and ACAP2 is recruited to the same compartment in a RAB35-dependent manner
  • in the nucleus, cytosol, and cell membrane
    • basic FUNCTION
  • GTPase activity
  • involved in vesicle transport
  • RAB35-regulated pathway plays an essential role during the terminal steps of cytokinesis by controlling septin and PIP2 subcellular distribution during cell division
  • TBC1D10C and RAB35 regulate a recycling pathway in T cells and contribute to immunological synapse (IS) formation, most likely by participating in TCR transport to the IS
  • playing a role in the regulation of cytoskeletal organization
  • play a role in recycling of the transferrin and other receptors and there is also evidence supporting a role for RAB35 in cytokinesis
  • might be involved in the formation of physiological extensions such as neurites
  • may be implicated in both cytokinesis, which involves remodelling of actin and tubulin cytoskeleton, and in endocytosis, which can involve interactions both with actin and with Rho-family GTPases
  • controls actin bundling by recruiting fascin as an effector protein
  • is a regulator of vesicle transport required specifically for phagocytosis
  • is the immune cell-specific regulator of vesicle transport within the actin-remodeling complex
  • localizes to the surface of oligodendroglia in a GTP-dependent manner, where it increases the density of vesicles, suggesting a function in docking or tethering
  • phosphocholination of RAB1A and RAB35 represents a mechanism by which bacterial FIC-domain-containing proteins can alter host-cell functions
  • is a critical upstream regulator of MICALL1 and ARF6, while both MICALL1 and ARF6 regulate RAB8A function
  • regulates endosomal membrane trafficking but also recruits effectors that modulate actin assembly and organization
  • RAB35 regulates cadherin-dependent adherens junctions (AJs) formation and myoblast fusion
  • key regulator of cargo recycling at endosomes, with an additional role in regulation of the actin cytoskeleton
  • RAB35 and its effector, ACAP2, a GTPase-activating protein that switches off ARF6 activity, negatively regulate oligodendrocyte morphological differentiation
  • regulates membrane trafficking from recycling endosomes to neurite tips during neurite outgrowth
  • molecular switch for membrane trafficking that regulates a variety of cellular events
  • small GTPase RAB35 functions as a molecular switch for membrane trafficking, specifically for endocytic recycling, by cycling between a GTP-bound active form and a GDP-bound inactive form
    • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • RAB35 forms a tripartite complex with MICALL1 and ACAP2 and recruits them to perinuclear ARF6-positive endosomes in response to nerve growth factor stimulation
    • INTERACTION
    DNA
    RNA
    small molecule nucleotide,
  • GTP
    • protein
  • RAB35 modulate TP53 transcriptional activity ofTP53RK
  • colocalizes with actin filaments and with CDC42, RAC1 and RHoA
  • OCRL is an effector of the RAB35 GTPase in cytokinesis abscission
  • RAB35 is a MICALL1-binding partner (over-expression of active RAB35 impairs the recruitment of MICALL1 to tubular recycling endosomes, whereas RAB35 depletion promotes enhanced MICALL1 localization to these structures)
  • likely RAB35 regulates actin-dependent phagosome formation by recruiting ACAP2, which might control actin remodeling and membrane traffic through ARF6
  • whereas DENND1A and DENND1B activate RAB35 for endosomal trafficking, DENND1C uniquely activates RAB35 for its role in actin regulation
  • interplay between RAB35 and ARF6 controls cargo recycling to coordinate cell adhesion and migration
  • regulates the formation of an EHD1-association site on ARF6-positive endosomes by integrating the functions of two distinct RAB35 effectors for successful neurite outgrowth
  • functions as a master RAB that determines the intracellular localization of MICALL1, which in turn functions as a scaffold for RAB8A, RAB13, and RAB36
  • CLIC4 is a regulator of RAB35 activity and serum- and LPA-dependent integrin trafficking
  • promotes neurite outgrowth of nerve growth factor-stimulated PC12 cells through interaction with ACAP2
  • RAB35 accumulates on bacteria-containing endosomes, and RAB35 directly binds and recruits CALCOCO2 to internalized bacteria
    • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS