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FLASH GENE

Symbol

WASF3

contributors: mct - updated : 14-11-2013

HGNC name	WAS protein family, member 3
HGNC id	12734

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	13q12.13      Physical location : 27.131.839 - 27.263.080
Synonym name	WASP family Verprolin-homologous protein 3
	wiskott-Aldrich syndrome protein family member 3
Synonym symbol(s)	WAVE3, SCAR3, KIAA0900, Brush-1

DNA

TYPE	functioning gene
STRUCTURE	131.24 kb     10 Exon(s)

10 Kb 5' upstream gene genomic sequence study

regulatory sequence	Promoter
text structure	promoter region contains HIF1A response elements (HRE)

MAPPING

cloned

Y

linked

N

status

provisional

Map	cen - D13S1244 - WASF3 - D13S1242 - D13S1246 - D13S290 - D13S260 - qter
Authors	Gene Map

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_006646 10 - 4850 NP_006637 - 502 - 2005 1627257

EXPRESSION

Type

widely

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Endocrine parathyroid       highly Nervous brain       moderately   nerve cranial nerve     highly Reproductive female system ovary

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Nervous central       Nervous peripherous

cell lineage

cell lines

fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	a N terminal leucine-zipper motif
	a highly basic region
	a VCA domain for activating Arp2/3
	a C terminal VPH (verproline homology)/WH2 (WASP homology 2) domain, binding to LDOC1

HOMOLOGY

Homologene

FAMILY

WAS protein family

CATEGORY

motor/contractile , structural protein

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,cytoplasm,cytoskeleton,microfilament

basic FUNCTION	play a major role in actin polymerization and cytoskeleton organization processes
	a possible involvement in the pathogenesis of neuroblastoma
	playing an important role in cell differentiation and motility
	regulate the remodeling of actin cytoskeleton downstream of Rho GTPases
	thought to be required for lamellipodium and ruffle formation in cells
	coordinate the development of lamellipodia at the leading edges of cells, and loss of its function prevents motility
	activation of WASF3 to promote actin remodeling is enhanced by the ABL-mediated tyrosine phosphorylation of WASF3
	is pivotal in controlling the invasiveness of prostate cancer cells
	required for invasion and metastasis in different cancer cell types

CELLULAR PROCESS	cell organization/biogenesis
	cell communication

PHYSIOLOGICAL PROCESS

text	cell shape and cell cycle control

PATHWAY

metabolism

signaling

a component

associating with the Arp2/3 complex a nucleating core constituent for actin polymerization in vitro

INTERACTION

DNA

RNA

small molecule

protein	dependence of WASF3 on HSP70 and HSP90 chaperone proteins for its function
	HSPA1A stabilizes the WASF3 protein
	is likely protected from proteasome degradation by HSPA1A
	WASF3 is a HIF1A-regulated gene
	novel signaling network whereby JAK2/STAT3 signaling creates a feed-forward loop to raise activated WASF3 levels that promote cancer cell motility
	ATAD3A interacts with the WASF3 metastasis-promoting protein
	stabilization of WASF3 function occurs through its interaction with ATAD3A and HSPA5, which may provide a bridge between the ER and mitochondria, allowing communication between the two organelles

cell & other

REGULATION

Phosphorylated by

ABL kinase that is essential for its ability to regulate invasion

Other

ABL1-mediated phosphorylation of WAVE3 is required for lamellipodia formation and cell migration

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral     --over   associated with more aggressive and metastatic stages of breast cancer

Susceptibility

Variant & Polymorphism

Candidate gene
Marker	potential marker for predicting tumor aggressiveness 6)
	marker for increased risk of breast-cancer-specific mortality and for the metastatic potential of the Triple Negative breast cancer cells
Therapy target
	System Type Disorder Pubmed cancer reproductive breast attractive therapeutic target for the treatment of metastatic breast cancer

ANIMAL & CELL MODELS