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FLASH GENE
Symbol RPA2 contributors: mct/pgu - updated : 02-03-2012
HGNC name replication protein A2, 32kDa
HGNC id 10290
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • a phosphorylated N terminal domain,
  • a central ssDNA-binding domain, and a DNA-binding domain DBD-D
  • a C terminal protein interaction domain, mediating interaction with XPA
    • HOMOLOGY
    Homologene
    FAMILY replication protein A family
    CATEGORY DNA associated
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,nucleus,nucleoplasm
    basic FUNCTION
  • colocalizing with RAD51 on synapsed axes in meiosis and facilitating RAD51 function, also colocalizing with BLM in meiotic prophase nuclei of spermatocytes
  • RPA2 phosphorylation facilitates chromosomal DNA repair
  • its hyperphosphorylation plays a critical role in maintenance of genomic stability and cell survival after a DNA replication block via promotion of homologous recombination
  • is an exchange point for multiple proteins involved in DNA repair, homologous recombination, and checkpoint processes and that it binds to different partners with comparable binding affinity using a single site
  • with RPA1, and RPA3, plays essential roles in DNA transactions
  • RPA1 and RPA2 are arranged from 5prime to 3prime along the unfolded telomeric G4, as already described for unstructured single-stranded DNA
    • CELLULAR PROCESS nucleotide, replication
    nucleotide, repair, nucleotide excision repair
    nucleotide, repair, recombination
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • component 70kDa of a trimeric single strand binding protein, the replication protein A complex, involved in replication, homologous recombination and nucleotide excision repair
  • MEIOB forms a complex with RPA2 and with SPATA22, and these three proteins co-localize in foci that are associated with meiotic chromosome
    • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • PPP4C dephosphorylates RPA2 to facilitate DNA repair via homologous recombination
  • RPA2 also interacts with TIPIN during the intra-S checkpoint
  • interacts with the specific proteins among those that play roles in the regulation of the replication fork progression
  • RFWD3 and RPA2 functionally interact and participate in replication checkpoint control
    • cell & other
    REGULATION
    Phosphorylated by RPA2 hyperphosphorylation by DNA-PK in response to double-strand breaks blocks unscheduled homologous recombination and delays mitotic entry
    ATR (in response to UV-induced DNA damage, ATR rapidly phosphorylates RPA2, disrupting its association with replication centers in the S-phase and contributing to the inhibition of DNA replication)
    Other dephosphorylated by the serine/threonine protein phosphatase 2A (PPP2R4)(PPP2R4-mediated RPA2 dephosphorylation is required for the efficient DNA damage repair)
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS