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FLASH GENE

Symbol

RPA2

contributors: mct/pgu - updated : 02-03-2012

HGNC name	replication protein A2, 32kDa
HGNC id	10290

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

DNA

TYPE	functioning gene
STRUCTURE	23.19 kb     9 Exon(s)

Genomic sequence alignment details
10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

N

status

confirmed

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_002946 9 - 1741 NP_002937 - 270 - 1999 10449415

EXPRESSION

Type

widely

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Digestive salivary gland       highly Endocrine parathyroid       highly Reproductive female system ovary     highly

cell lineage	spermatocytes
cell lines
fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	a phosphorylated N terminal domain,
	a central ssDNA-binding domain, and a DNA-binding domain DBD-D
	a C terminal protein interaction domain, mediating interaction with XPA

HOMOLOGY

Homologene

FAMILY

replication protein A family

CATEGORY

DNA associated

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,nucleus,nucleoplasm

basic FUNCTION	colocalizing with RAD51 on synapsed axes in meiosis and facilitating RAD51 function, also colocalizing with BLM in meiotic prophase nuclei of spermatocytes
	RPA2 phosphorylation facilitates chromosomal DNA repair
	its hyperphosphorylation plays a critical role in maintenance of genomic stability and cell survival after a DNA replication block via promotion of homologous recombination
	is an exchange point for multiple proteins involved in DNA repair, homologous recombination, and checkpoint processes and that it binds to different partners with comparable binding affinity using a single site
	with RPA1, and RPA3, plays essential roles in DNA transactions
	RPA1 and RPA2 are arranged from 5prime to 3prime along the unfolded telomeric G4, as already described for unstructured single-stranded DNA

CELLULAR PROCESS	nucleotide, replication
	nucleotide, repair, nucleotide excision repair
	nucleotide, repair, recombination

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

a component	component 70kDa of a trimeric single strand binding protein, the replication protein A complex, involved in replication, homologous recombination and nucleotide excision repair
	MEIOB forms a complex with RPA2 and with SPATA22, and these three proteins co-localize in foci that are associated with meiotic chromosome

INTERACTION

DNA

RNA

small molecule

protein	PPP4C dephosphorylates RPA2 to facilitate DNA repair via homologous recombination
	RPA2 also interacts with TIPIN during the intra-S checkpoint
	interacts with the specific proteins among those that play roles in the regulation of the replication fork progression
	RFWD3 and RPA2 functionally interact and participate in replication checkpoint control

cell & other

REGULATION

Phosphorylated by	RPA2 hyperphosphorylation by DNA-PK in response to double-strand breaks blocks unscheduled homologous recombination and delays mitotic entry
	ATR (in response to UV-induced DNA damage, ATR rapidly phosphorylates RPA2, disrupting its association with replication centers in the S-phase and contributing to the inhibition of DNA replication)

Other

dephosphorylated by the serine/threonine protein phosphatase 2A (PPP2R4)(PPP2R4-mediated RPA2 dephosphorylation is required for the efficient DNA damage repair)

ASSOCIATED DISORDERS

ANIMAL & CELL MODELS