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FLASH GENE
Symbol RPA2 contributors: mct/pgu - updated : 02-03-2012
HGNC name replication protein A2, 32kDa
HGNC id 10290
Location 1p35.3      Physical location : 28.218.048 - 28.241.236
Synonym name
  • replication protein A, subunit 2
  • RF-A protein 2
  • Synonym symbol(s) REPA2, RPA32, RPA34, RP-A p32
    DNA
    TYPE functioning gene
    STRUCTURE 23.19 kb     9 Exon(s)
    Genomic sequence alignment details
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked N status confirmed
    Physical map
    NUDC 1p35-p34 nuclear distribution gene C homolog (A. nidulans) FLJ34633 1p35.3 hypothetical protein FLJ34633 LOC391022 1 similar to DC2 protein LOC388610 1 LOC388610 MGC16491 1p35.3 hypothetical protein MGC16491 SLC9A1 1p35 solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive) LOC391023 1 similar to ribosomal protein L18a; 60S ribosomal protein L18a LOC284609 1p35.3 similar to hypothetical protein FLJ20420 LOC148356 1p35.3 similar to nucleophosmin 1; nucleolar phosphoprotein B23; numatrin; nucleophosmin/nucleoplasmin family, member 1 WDTC1 1p35.3 WD and tetratricopeptide repeats 1 DKFZP564D0478 1p35.3 hypothetical protein DKFZp564D0478 JFC1 1p35.3 hypothetical protein DKFZp564D0478 MAP3K6 1p35.2 mitogen-activated protein kinase kinase kinase 6 FCN3 1p36-p32 ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen) LOC388611 1 similar to EAPG6122 GPR3 1p35.1-p34.3 G protein-coupled receptor 3 WASF2 1p36.11-p34.3 WAS protein family, member 2 DJ159A19.3 1p36.13 hypothetical protein DJ159A19.3 LOC388612 1 LOC388612 FGR 1p35 Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog G1P3 1p35 interferon, alpha-inducible protein (clone IFI-6-16) LOC339409 1p35.3 similar to Procollagen (type III) N-endopeptidase MGC34648 1p35.3 hypothetical protein MGC34648 STX12 1p35-p34.1 syntaxin 12 PPP1R8 1p35 protein phosphatase 1, regulatory (inhibitor) subunit 8 C1orf38 1p35.2 chromosome 1 open reading frame 38 RPA2 1p35 replication protein A2, 32kDa ASML3B 1p35.2 acid sphingomyelinase-like phosphodiesterase FLJ10307 1p35.2 hypothetical protein FLJ10307 EYA3 1p36 eyes absent homolog 3 (Drosophila) LOC388613 1 similar to Microsomal signal peptidase 25 kDa subunit (SPase 25 kDa subunit) (SPC25) LOC391024 1 similar to RIKEN cDNA 2610313E07 PTAFR 1p35.1-p34.3 platelet-activating factor receptor DNAJC8 1p35.3 DnaJ (Hsp40) homolog, subfamily C, member 8 ATPIF1 1p35.3 ATPase inhibitory factor 1 SESN2 1p35.3 sestrin 2 FLJ20045 1p35.2 TRAP/mediator complex subunit p28b FLJ13171 1p35 TRAP/mediator complex subunit p28b CHC1 1p36.1 chromosome condensation 1 SECP43 1p35.2 tRNA selenocysteine associated protein LOC85028 1p35.2 PNAS-123 MGC45806 1p35.2 hypothetical protein MGC45806 TAF12 1p35.2 TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa GMEB1 1p35.2 glucocorticoid modulatory element binding protein 1
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    9 - 1741 - 270 - 1999 10449415
    EXPRESSION
    Type widely
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestivesalivary gland   highly
    Endocrineparathyroid   highly
    Reproductivefemale systemovary  highly
    cell lineage spermatocytes
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a phosphorylated N terminal domain,
  • a central ssDNA-binding domain, and a DNA-binding domain DBD-D
  • a C terminal protein interaction domain, mediating interaction with XPA
  • HOMOLOGY
    Homologene
    FAMILY replication protein A family
    CATEGORY DNA associated
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,nucleus,nucleoplasm
    basic FUNCTION
  • colocalizing with RAD51 on synapsed axes in meiosis and facilitating RAD51 function, also colocalizing with BLM in meiotic prophase nuclei of spermatocytes
  • RPA2 phosphorylation facilitates chromosomal DNA repair
  • its hyperphosphorylation plays a critical role in maintenance of genomic stability and cell survival after a DNA replication block via promotion of homologous recombination
  • is an exchange point for multiple proteins involved in DNA repair, homologous recombination, and checkpoint processes and that it binds to different partners with comparable binding affinity using a single site
  • with RPA1, and RPA3, plays essential roles in DNA transactions
  • RPA1 and RPA2 are arranged from 5prime to 3prime along the unfolded telomeric G4, as already described for unstructured single-stranded DNA
  • CELLULAR PROCESS nucleotide, replication
    nucleotide, repair, nucleotide excision repair
    nucleotide, repair, recombination
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • component 70kDa of a trimeric single strand binding protein, the replication protein A complex, involved in replication, homologous recombination and nucleotide excision repair
  • MEIOB forms a complex with RPA2 and with SPATA22, and these three proteins co-localize in foci that are associated with meiotic chromosome
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • PPP4C dephosphorylates RPA2 to facilitate DNA repair via homologous recombination
  • RPA2 also interacts with TIPIN during the intra-S checkpoint
  • interacts with the specific proteins among those that play roles in the regulation of the replication fork progression
  • RFWD3 and RPA2 functionally interact and participate in replication checkpoint control
  • cell & other
    REGULATION
    Phosphorylated by RPA2 hyperphosphorylation by DNA-PK in response to double-strand breaks blocks unscheduled homologous recombination and delays mitotic entry
    ATR (in response to UV-induced DNA damage, ATR rapidly phosphorylates RPA2, disrupting its association with replication centers in the S-phase and contributing to the inhibition of DNA replication)
    Other dephosphorylated by the serine/threonine protein phosphatase 2A (PPP2R4)(PPP2R4-mediated RPA2 dephosphorylation is required for the efficient DNA damage repair)
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS