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FLASH GENE

Symbol

UBE2E3

contributors: shn/npt - updated : 21-03-2015

HGNC name	ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)
HGNC id	12479

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	2q31.3      Physical location : 181.845.111 - 181.928.150
Synonym name	ubiquitin-protein ligase E3
	ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5)
	ubiquitin carrier protein E3
Synonym symbol(s)	UbcH9, UbcM2, UBCE4
EC.number	6.3.2.19

DNA

TYPE	functioning gene
STRUCTURE	83.04 kb     7 Exon(s)

10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

status

provisional

Map	cent - D2S2261 - D2S384 - UBE2E3 - D2S2310 - D2S364 - qter

RNA

TRANSCRIPTS	type	messenger

text	Two alternatively spliced transcript variants encoding the same protein have been found for this gene

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_006357 6 - 1.559 NP_006348 23 207 - 1999 10343118

EXPRESSION

Type	ubiquitous

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Cardiovascular heart       highly Digestive salivary gland       highly Endocrine parathyroid       highly

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Muscular striatum skeletal   highly

cell lineage
cell lines	telomerase-imortalized RPE cells (RPE-1)
fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	a conserved UBC domain of 150AA harboring the cysteine residue required for enzyme-ubiquitin thioester formation
	U-box and RING domains

HOMOLOGY

interspecies	homolog to yeast UBC4/5
	ortholog to Ube2e3, Mus musculus
	ortholog to Ube2e3, rattus norvegicus

Homologene

FAMILY

E2 ubiquitin-conjugating enzyme family

CATEGORY

enzyme

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,cytoplasm
	intracellular,nucleus
text	is potentially imported into nuclei by the transport receptor importin-11 (IPO11)

basic FUNCTION	catalyzes the covalent attachment of ubiquitin to other proteins
	participates in the regulation of transepithelial sodium transport in renal cells
	May be involved in cell growth arrest
	shuttles between the nuclear and cytoplasmic compartments
	modulates the balance between RPE cell proliferation and differentiation
	in concert with substrate adaptors, engages activated CUL3 ligases, thus suggesting that class III E2s are novel regulators of cullin ligases
	play an essential role in the proliferation of RPE cells
	can enhance the transcriptional activity of endogenous NFE2L2 and Cys-136 and the active-site cysteine, Cys-145, jointly contribute to this regulation
	implicated in the restoration of redox homeostasis following oxidative stress

CELLULAR PROCESS	cell cycle
	cell life, proliferation/growth
	protein, degradation

PHYSIOLOGICAL PROCESS

text	protein modification, protein ubiquitination

PATHWAY

metabolism

signaling

a component	UBE2E3-RCBTB1 complex led to the discovery and characterization of the following novel interactions
	UBE2E3 and NFE2L2 form a nuclear complex utilizing the DNA binding, Neh1 domain, of NFE2L2

INTERACTION

DNA

RNA

small molecule

protein	IPO11
	NEDD4L
	binds an N-terminal domain of CUL3 requiring the first 57 amino acids, the same domain that binds to RCBTB1 and other substrate adaptors
	interacting with CUL3 independent of a bridging RING-finger protein and can engage the neddylated (i.e., activated) form of CUL3
	ubiquitin-conjugating enzyme that is a novel regulator of NFE2L2
	bind both E2&
	8764; ubiquitin conjugates and substrates to facilitate transfer of the ubiquitin molecule
	MUL1 is involved in multiple biological pathways, and the interaction of GABARAP with MUL1-UBE2E3 supports the role of MUL1 as an important regulator of mitophagy and provides a plausible mechanism for its function in this process

cell & other

REGULATION

ASSOCIATED DISORDERS

ANIMAL & CELL MODELS