|
| Transgenic mice, in which both NP1 and NP2 were targeted died in utero at E8.5  | |
Mice deficient for NP2 but heterozygous for NP1 or deficient for NP1 but heterozygous for NP2 were also embryonic lethal and survived to E10-E10.5 |
|
Morpholino-mediated knockdown of zNRP1 in embryos resulted in vascular defects, most notably impaired circulation in the intersegmental vessels |
|
Overexpression of Y297A and D320A NRP1 mutations in human umbilical vein endothelial cells reduced high-affinity VEGF binding and migration toward a VEGF gradient, and markedly inhibited VEGF-induced angiogenesis in a coculture cell model |