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FLASH GENE
Symbol SF1 contributors: mct - updated : 16-10-2018
HGNC name splicing factor 1
HGNC id 12950
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • a nuclear localization signal
  • a K homology (KH) motif bound to a RNA binding motif
  • a CCHC-type zinc finger domain
  • a conserved SPSP motif in a coiled-coil domain of SF1 is highly phosphorylated in proliferating human cells and is required for cell proliferation
  • a C terminal glutamine and proline rich domain
  • conjugated PhosphoP , Other
    mono polymer complex
    HOMOLOGY
    interspecies homolog to rattus Zfp162 (96.8 pc)
    homolog to murine Sf1 (99.2 pc)
    Homologene
    FAMILY
  • zinc finger protein family
  • BBP/SF1 family
  • CATEGORY regulatory , transcription factor
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,cytosolic,ribosome
    intracellular,nucleus,nucleoplasm,nuclear bodies,nuclear speckles
    text
  • distributed throughout the nucleoplasm, where transcription occurs, with additional concentration in nuclear speckles, where splicing factors accumulate when not engaged in splicing
  • basic FUNCTION
  • being necessary for the ATP-dependent first step of spliceosome assembly
  • potentially involved in regulating RNA cellular metabolism
  • may act as transcription repressor
  • SF1 and U2AF2 associate cooperatively with pre-mRNA and play a crucial role in 3 prime splice site recognition during early steps of spliceosome assembly
  • RNA polymerase II transcription factor activity
  • role for SF1 and branch site-like sequences in splice site selection
  • SF1 and U2 auxiliary factor (U2AF2) cooperatively recognize the 3 prime splice site during the initial stages of pre-mRNA splicing
  • represses proinflammatory gene expression in vascular smooth muscle cells (SMCs) at a global level and thus may also be involved in the attenuation of growth factor-induced phenotype changes in these cells
  • the essential splicing factors U2AF2 and SF1 cooperatively bind consensus sequences at the 3' end of introns
  • CELLULAR PROCESS nucleotide, transcription, maturation
    nucleotide, transcription, regulation
    nucleotide, RNA splicing
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • acetylated
  • spliceosome complex
  • SF1 and U2AF form extraspliceosomal complexes before and after taking part in the assembly of catalytic spliceosomes
  • INTERACTION
    DNA binding
    RNA
  • interacting with U1 snRNA
  • binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA
  • small molecule metal binding,
  • Zn2+
  • protein
  • binding to the RRM3 motif of U2AF65
  • binding to EWSR1
  • binding to FUS
  • binding to TAF15
  • binding to U2AF2
  • direct binding of SF1 to U2AF2 in both the nucleoplasm
  • and nuclear speckles
  • TRAPPC2 interaction with the transcription factors ENO1, PITX1 and SF1
  • antagonizes PDGFB-induced growth and dedifferentiation of vascular smooth muscle cells
  • PRPF40B interacts directly with SF1 and associates with U2AF2
  • U2AF2 interacts with SF1 and was shown to recruit the U2 snRNP to the spliceosome
  • interacts with SURP domains of U2 snRNP-associated proteins
  • cell & other
    REGULATION
    induced by CSF2 (forms ABCDF and B4)
    inhibited by phosphorylation of ser20, interfering with U2AF65 binding and spliceosome assembly
    Phosphorylated by UHMK1, that double-phosphorylates both serines of the SPSP motif
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS