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FLASH GENE

Symbol

TRIM27

contributors: mct - updated : 30-04-2016

HGNC name	tripartite motif-containing 27
HGNC id	9975

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

DNA

TYPE	functioning gene
STRUCTURE	20.99 kb     8 Exon(s)

MAPPING

cloned

Y

linked

status

provisional

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_006510 8 - 2969 NP_006501 58.5 513 - 2013 23452853 RFP variant alpha,isoform alpha distinct C terminus RFP variant beta,isoform beta - - 2700 40.8 358 - 2013 23452853

EXPRESSION

Type

widely

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Endocrine parathyroid       highly Lymphoid/Immune lymph node       highly Urinary bladder       highly

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Connective adipose     highly

cell lineage

cell lines

fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	a N terminal RING zinc
	two B boxes domains linked to a leucine coiled-coil domain (RBCC) the so called tripartite domain (TRIM)
	a C terminal B30-2-like (RFP) domain, also a SPRY domain

conjugated

MetalloP

HOMOLOGY

Homologene

FAMILY	B box family, RBCC (TRIM family)
	C-IV TRIM family

CATEGORY

transcription factor , protooncogene

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,cytoplasm,organelle,membrane
	intracellular,cytoplasm,organelle,endosome
	intracellular,nucleus,nucleoplasm,nuclear bodies
text	contained in nuclear bodies (NB) associated with the nuclear matrix

basic FUNCTION	activate with MCRS1 and CHD4 ribosomal gene transcription
	is a previously undescribed negative regulator of CD4 T cells
	is an important negative regulator of mast cells
	TRIM27 affects NOD2-mediated pro-inflammatory responses
	NOD2 and TRIM27 might functionally cooperate in the nucleus
	tripartite motif (TRIM) protein containing, positively regulating TNF-induced apoptosis
	works as a novel E3 ligase in the PAX7-mediated degradation of MYOD1 in response to skeletal muscle atrophy

CELLULAR PROCESS	cell life, differentiation
	nucleotide, transcription
	cell organization/biogenesis

PHYSIOLOGICAL PROCESS

text	spermatogenesis
	differentiation of male germ cells

PATHWAY

metabolism

signaling

a component	E3 RING ubiquitin ligase, MAGEL2-TRIM27, localizes to endosomes through interactions with the retromer complex
	ubiquitin ligase activity of MAGEL2-TRIM27 is important for proper retrograde transport
	facilitates ITGA5 recycling, an important event in tumorigenesis
	complexing with USP7 and ubiquitination-deubiquitination cascade mediated by the TRIM27-USP7 complex plays an important role in TNF-induced apoptosis
	ZNF446, and its associated tripartite motif protein, TRIM27, are obligate components of the ZNF165-SMAD3 complex that also support tumor cell viability

INTERACTION

DNA	binding to the enhancer of polycomb protein (EPC1)

RNA

small molecule	metal binding,
	heavy metal
	Zn2+

protein	interacting with CHD4 (involved in transcriptional repression in the nucleus, co-localize with MCRS1 in the nucleolus and appear to activate the rRNA transcription)
	functions to negatively regulate KCNN4 channel activity and TCR-stimulated Ca2+ influx and cytokine production in activated CD4 T cells
	TRIM27 functions as an E3 ligase and mediates lysine 48 polyubiquitination of PI3KC2B, leading to a decrease in PI3K enzyme activity
	is a transcriptional repressor that interacts with, and attenuates senescence induction by, the retinoblastoma-associated protein (RB1)
	TRIM27 functions as an E3 ligase to ubiquitinate and inhibit PI3KC2B kinase activity
	both PI3KC2B and TRIM27 play critical but opposite roles in FCER1A-stimulated activation of KCNN4, Ca2+ influx, degranulation, and cytokine production in bone marrow mast cells
	TRIM27 is a new specific binding partner for NOD2
	TRIM27 was identified as a major binding partner of MAGEL2 and MAGEL2-TRIM27 is likely required for endosome-to-Golgi retrograde transport
	works as an E3 ligase in PAX7-induced degradation of MYOD1
	SIGLEC1 suppresses antiviral innate immune response by inducing TBK1 degradation via the ubiquitin ligase TRIM27

cell & other

REGULATION

repressed by

IFN-gamma and LPS

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral fusion       TRIM-RET fusion in thyroid cancer constitutional     --over   in Crohn disease patients

Susceptibility

Variant & Polymorphism

Candidate gene
Marker
Therapy target
	System Type Disorder Pubmed immunology inflammatory   could be a new target for therapeutic intervention in NOD2-associated diseases

ANIMAL & CELL MODELS

Trim27-/- mice are more susceptible to acute anaphylaxis