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FLASH GENE
Symbol PIF1 contributors: mct - updated : 11-06-2012
HGNC name PIF1 5'-to-3' DNA helicase homolog
HGNC id 26220
RNA
TRANSCRIPTS type messenger
identificationnb exonstypebpproduct
ProteinkDaAAspecific expressionYearPubmed
13 - 2659 - 641 - 2010 20524933
EXPRESSION
Type
   expressed in (based on citations)
organ(s)
cell lineage
cell lines
fluid/secretion
at STAGE
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • a unique N-terminal portion, named the PIF1 N-terminal (PINT) domain, and when the PINT domain was truncated, apparent affinity for ssDNA and unwinding activity were much reduced
    • HOMOLOGY
    Homologene
    FAMILY DNA helicase family
    CATEGORY DNA associated
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,nucleus,chromatin/chromosome,telomere
    basic FUNCTION
  • functions of PIF1 might be restricted with particular situations and DNA structures
  • specifically recognizes and unwinds DNA structures resembling putative stalled replication forks
  • implicated in DNA replication processes such as Okazaki fragment maturation and replication fork pausing
  • G4 DNA-binding and resolvase protein
  • could have a role in processing G4 structures that arise in the single-stranded nucleic acid intermediates formed during DNA replication and gene expression
  • PIF1 and EXO1 resect telomeric DNA <5 kb from the chromosome end, stimulating weak checkpoint activation; resection is extended >5 kb by EXO1 and full checkpoint activation occurs
  • 5prime->3prime DNA helicase that exhibits broad substrate specificity
  • potential roles for PIF1 in S-phase entry and progression that are essential to protect human tumor cells from apoptosis
    • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    alternative pathway in which PIF1 removes Okazaki fragments initiated by fold-back flaps
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • interacting wit telomerase (telomerase is essential to alleviate PIF1-induced replication stress at telomeres)
  • preferentially bound synthetic G4 DNA relative to ssDNA (single-stranded DNA), dsDNA (double-stranded DNA) and a partially single-stranded duplex DNA helicase substrate
    • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS