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FLASH GENE

Symbol

TRIM50

contributors: mct - updated : 17-03-2017

HGNC name	tripartite motif-containing 50
HGNC id	19017

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

DNA

TYPE	functioning gene
STRUCTURE	15.55 kb     7 Exon(s)

10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

N

status

provisional

Map	cen - D7S653 - D7S672 - TRIM35 - D7S2476 - D7S2472 - qter
Authors	UCSC (2009)

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_178125 7 - 2050 NP_835226 54.6 487 - 2014 24308962 NM_001281450 7 - 2047 NP_001268379 - 486 - 2014 24308962 NM_001281451 7 - 1978 NP_001268380 - 487 - 2014 24308962

EXPRESSION

Type	ubiquitous

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Digestive intestine       highly   liver       highly   mouth tongue     highly   stomach mucous gland     highly Homo sapiens Nervous brain       highly

cells

System Cell Pubmed Species Stage Rna symbol Digestive gastric Homo sapiens

cell lineage

cell lines

fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	a RING-type zinc finger
	a B box-type zinc finger
	a coiled-coil domain
	a B30.2/SPRY domain

HOMOLOGY

interspecies	homolog to rattus Trim50 (87.6 pc)
	homolog to murine Trim50 (89.1 pc)

intraspecies

homolog to TRIM72

Homologene

FAMILY	TRIM/RBCC family
	C-IV TRIM family

CATEGORY

enzyme , regulatory

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,cytoplasm,cytosolic
text	TRIM50 resides likely in the cell interior canaliculi generated by tubulovesicular fusion during the stimulated phase

basic FUNCTION	acting as a E3-ubiquitin-protein ligase
	catalyzing uquitination in presence of UBE2E2 through the Ring domain
	TRIM50 localizes to and promotes the recruitment and aggregation of polyubiquitinated proteins to the aggresome
	likely when the proteasome activity is impaired, TRIM50 fails to drive its substrates to the proteasome-mediated degradation, and promotes their storage in the aggresome for successive clearance
	is likely involved in vesicular dynamics by sensing the phosphorylated state of phosphoinositol lipids
	seems to play an essential role in tubulovesicular dynamics, promoting the formation of sophisticated canaliculi and microvilli during acid secretion in gastric parietal cells
	TRIM50 and TRIM72 may exert similar biological roles in vesicular trafficking below the cell membrane in muscle and parietal cells, respectively
	TRIM50 promotes the formation and clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction, a tubulin specific deacetylase that regulates microtubule-dependent aggresome formation

CELLULAR PROCESS

protein, ubiquitin dependent proteolysis

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

a component

INTERACTION

DNA

RNA

small molecule	metal binding,
	Zn2+

protein	TRIM50 colocalizes, interacts with and increases the level of SQSTM1, a multifunctional adaptor protein implicated in various cellular processes including the autophagy clearance of polyubiquitinated protein aggregates
	TRIM50 may share similar biological functions with TRIM72 in vesicular trafficking
	EP300 and KAT2B are two TRIM50 acetyltransferases and the balance between ubiquitination and acetylation regulates TRIM50 degradation

cell & other

REGULATION

ASSOCIATED DISORDERS

corresponding disease(s)

WBS

Susceptibility

Variant & Polymorphism

Candidate gene

Marker

Therapy target

ANIMAL & CELL MODELS

Trim50 knock-out mice retained normal histology in the gastric mucosa but exhibited impaired secretion of gastric acid