| protein
| interacts preferentially with GDP-bound RAB21 and has a much stronger guanine nucleotide exchange activity towards RAB21 than RAB5A |
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RAB38 is a ANKRD27-associated protein (physically interacts with RAB38, and preferentially binds to the active GTP-bound form of RAB38) |
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RAB38 effector, that might also be involved in targeting TYRP1 or/and tyrosinase to the melanosome for the synthesis of pigment |
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VAMP7 interacts with ANKRD27, a guanine nucleotide exchange factor (GEF) of the small GTPase RAB21, through a specific domain herein called the interacting domain (ID)  |
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specifically binds two small GTPases, RAB32 and RAB38, which redundantly regulate the trafficking of melanogenic enzymes in mammalian epidermal melanocytes |
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ANKRD27-VAMP7 interaction is essential for the peripheral melanosomal distribution of TYRP1 in melanocytes |
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recruitment of ANKRD27 to the endosomal membrane is mediated by its direct interaction with VPS29, a subunit of the retromer complex, which is involved in trafficking from endosomes to the TGN and the cell surface |
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suppression of VPS35 led to mistrafficking of the melanogenic enzymes, tyrosinase and TYRP1, establishing that retromer acts in concert with ANKRD27 in this trafficking pathway |
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in addition to VAMP7, ANKRD27 binds to RAB32/38 |
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HPS4 regulates recycling of VAMP7 from melanosomes, most likely by recruiting RAB38 and consequently ANKRD27 to initiate and/or complete tubule formation |
