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FLASH GENE

Symbol

CHAF1B

contributors: SGE/npt - updated : 26/04/2010

HGNC name	chromatin assembly factor 1, subunit B (p60)
HGNC id	1911

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

DNA

TYPE	functioning gene
STRUCTURE	31.44 kb     14 Exon(s)

10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

status

confirmed

Map	cen - CBR1 - D21S333 - CHAF1B - D21S334 - SIM2 /(D21S396 ,D21S395 ) - D21S167 - D21S335 - qter
Authors	Blouin (96)

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_005441 14 - 2297 NP_005432 - 559 - Kaufman (1995)

EXPRESSION

Type

widely

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Digestive esophagus       highly Nervous brain           nerve       highly Reproductive male system prostate       Respiratory lung

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Blood / hematopoietic bone marrow     highly Muscular striatum skeletal

cell lineage

cell lines

fluid/secretion

at STAGE

cell cycle

cell cycle, G1, S, G2, M

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains

7 WD-40 repeat domains

conjugated

PhosphoP

HOMOLOGY

interspecies	homolog to murine Chaf1b (84.4pc)
	homolog to rattus Chaf1b (84.8pc)

Homologene

FAMILY

WD-repeat HIR1 family

CATEGORY

chaperone/stress

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,cytoplasm
	intracellular,nucleus
text	normally found in the nucleus except during mitosis, when it is released into the cytoplasm

basic FUNCTION	required for the assembly of histone octamers onto newly-replicated DNA
	p60 subunit required for chromatin assembly after replication and may also be involved in DNA repair
	essential factor not only for S-phase-specific chromatin assembly but also for proliferating cell viability
	recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases

CELLULAR PROCESS

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

a component	component of a complex composed of p50, p60, and p150, active complex in G1, S and G2 phases
	associating with SETDB1-CBX5 chaperone complex and monomethylating K9 on non nucleosomal histone H3, thus providing H3K9me1 for subsequent trimethylation by SUV39H1/H2 in pericentric regions (Loyola 2009)

INTERACTION

DNA

RNA

small molecule

protein

cell & other

REGULATION

Other

hyperphosphorylated during mitosis and released in the cytoplasm

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function constitutional     --low   in Down syndrome

Susceptibility

Variant & Polymorphism

Candidate gene	overexpression of CAF-1/p60 characterized prostatic cancers with a worse prognosis (Staibano 2009)
Marker
Therapy target

ANIMAL & CELL MODELS