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FLASH GENE
Symbol CHAF1B contributors: SGE/npt - updated : 26/04/2010
HGNC name chromatin assembly factor 1, subunit B (p60)
HGNC id 1911
Location 21q22.12-q22.13      Physical location : 37.757.688 - 37.789.124
Synonym name
  • CAF-I 60 kDa subunit
  • M-phase phosphoprotein 7
  • chromatin assembly factor 1 subunit B
  • CAF-1 subunit B
  • Synonym symbol(s) CAF1AP60, CAF1A, CAFB, MPHOSPH7, MPP7, CAF1, CAF-1, CAF1P60, CAF-IP60
    DNA
    TYPE functioning gene
    STRUCTURE 31.44 kb     14 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked   status confirmed
    Map cen - CBR1 - D21S333 - CHAF1B - D21S334 - SIM2 /(D21S396 ,D21S395 ) - D21S167 - D21S335 - qter
    Authors Blouin (96)
    Physical map
    RPL34P3 21q22.12 ribosomal protein L34 pseudogene 3 LOC266693 21q22.2 enhancer of zeste homolog 2 (Drosophila) pseudogene RPS20P1 21q22.12 ribosomal protein S20, pseudogene 1 PPP1R2P2 21q22.13 protein phosphatase 1, regulatory (inhibitor) subunit 2 pseudogene 2 RPL23AP3 21q22.2 ribosomal protein L23a pseudogene 3 C21orf18 21q22.13 chromosome 21 open reading frame 18 RIMKLP 21q22.13 ribosomal modification protein rimK-like (E. coli) pseudogene CBR1 21q22.2 carbonyl reductase 1 LOC388822 21 similar to Protein CGI-27 (C21orf19-like protein) LOC391280 21 similar to ribosomal protein S9 CBR3 21q22.2 carbonyl reductase 3 C21orf5 21q22.2 chromosome 21 open reading frame 5 RPL3P1 21q22.2 ribosomal protein L3 pseudogene 1 ZCWCC3 21q22.13 zinc finger, CW-type with coiled-coil domain 3 CHAF1B 21q22.2 chromatin assembly factor 1, subunit B (p60) CLDN14 21q22.3 claudin 14 PSMD15 21q22.13 proteasome (prosome, macropain) 26S subunit, non-ATPase, 15 LOC388823 21 LOC388823 SIM2 21q22.2 single-minded homolog 2 (Drosophila) HLCS 21q22.2 holocarboxylase synthetase (biotin-[proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) MRPL20P1 21q22.2 holocarboxylase synthetase (biotin-[proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) DSCR6 21q22.2 Down syndrome critical region gene 6 DSCR5 21q22.2 Down syndrome critical region gene 5 TTC3 21q22.2 tetratricopeptide repeat domain 3 DSCR9 21q22.13 Down syndrome critical region gene 9 DSCR3 21q22.3 Down syndrome critical region gene 3
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    14 - 2297 - 559 - Kaufman (1995)
    EXPRESSION
    Type widely
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestiveesophagus   highly
    Nervousbrain    
     nerve   highly
    Reproductivemale systemprostate   
    Respiratorylung    
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Blood / hematopoieticbone marrow  highly
    Muscularstriatumskeletal  
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    cell cycle     cell cycle, G1, S, G2, M
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • 7 WD-40 repeat domains
  • conjugated PhosphoP
    HOMOLOGY
    interspecies homolog to murine Chaf1b (84.4pc)
    homolog to rattus Chaf1b (84.8pc)
    Homologene
    FAMILY
  • WD-repeat HIR1 family
  • CATEGORY chaperone/stress
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm
    intracellular,nucleus
    text normally found in the nucleus except during mitosis, when it is released into the cytoplasm
    basic FUNCTION
  • required for the assembly of histone octamers onto newly-replicated DNA
  • p60 subunit required for chromatin assembly after replication and may also be involved in DNA repair
  • essential factor not only for S-phase-specific chromatin assembly but also for proliferating cell viability
  • recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • component of a complex composed of p50, p60, and p150, active complex in G1, S and G2 phases
  • associating with SETDB1-CBX5 chaperone complex and monomethylating K9 on non nucleosomal histone H3, thus providing H3K9me1 for subsequent trimethylation by SUV39H1/H2 in pericentric regions (Loyola 2009)
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
    cell & other
    REGULATION
    Other hyperphosphorylated during mitosis and released in the cytoplasm
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    constitutional     --low  
    in Down syndrome
    Susceptibility
    Variant & Polymorphism
    Candidate gene overexpression of CAF-1/p60 characterized prostatic cancers with a worse prognosis (Staibano 2009)
    Marker
    Therapy target
    ANIMAL & CELL MODELS