motifs/domains
| an N-terminal E2-like fold (ELF domain), |
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three potential WD40 repeats required for interaction with other subunits of the FA complex |
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a PHD-type zinc finger motif required for FANCD2 mono-ubiquitination and sharing sequence similarity to the canonical RING finger of c-CBL, including a conserved tryptophan required for E2 binding by c-CBL |
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a central domain (URD) dispensable for interaction with UBE2T but is essential to bind substrates FANCD2 and FANCI, but appears to be essential for the function of FANCL |
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a novel double-RWD (DRWD) domain, and a C-terminal RING domain predicted to facilitate E2 binding, and both necessary and sufficient for FANCL binding of UBE2T |
basic FUNCTION
| having ubiquitin ligase activity and may be the long -sought enzyme responsible for monoubiquitination of FANCD2 |
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required to prevent accumulation of replication-associated DNA double-strand breaks |
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via its WD40 region, binds the FA complex and, via its PHD, recruits an as-yet-unidentified E2 for mono-ubiquitination of FANCD2 |
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increases the activity and expression of CTNNB, a key pluripotency factor in hematopoietic stem cells |
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FANCA, FANCF, FANCL, FANCD2, BRCA1, and BRCA2, are required for mitophagy |