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FLASH GENE
Symbol EDF1 contributors: mct - updated : 21-08-2013
HGNC name endothelial differentiation-related factor 1
HGNC id 3164
Location 9q34.3      Physical location : 139.756.570 - 139.760.738
Synonym name multiprotein bridging factor 1
Synonym symbol(s) MBF1, EDF-1
DNA
TYPE functioning gene
STRUCTURE 4.22 kb     6 Exon(s)
10 Kb 5' upstream gene genomic sequence study
regulatory sequence Promoter (CAAT box)
Binding site   transcription factor
text structure
  • two GC boxes
  • binding sites for SP1/SP3 and NFY
    • MAPPING cloned Y linked N status provisional
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    5 - 701 16.2 148 - 2002 12040021
  • variant alpha
    • 4 splicing 700 15.1 139 - 2002 12040021
  • variant beta
  • differing in the 3'utr and coding region
    • 5 - 638 - 127 - 2002 12040021
    5 - 647 - 130 - 2002 12040021
    5 - 852 - 141 - 2002 12040021
    EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Cardiovascularheart    
    Digestiveliver    
    Endocrinepancreas    
    Nervousbrain    
    Respiratorylung    
    cells
    SystemCellPubmedSpeciesStageRna symbol
    Cardiovascularendothelial cell
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    physiological period fetal
    cell cycle     cell cycle
    Text kidney
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a N terminal region binding to the activators
  • a MBF1 C terminal domain for binding to TBP
    • HOMOLOGY
    interspecies homolog to murine Edf1 (98.6pc)
    homolog to chicken EDF1 (93.2pc)
    Homologene
    FAMILY
    CATEGORY signaling
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,nucleus,chromatin/chromosome
    intracellular,nucleus,nucleolus
    basic FUNCTION
  • bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery
  • transcriptional coactivator for transcription factors such as FTZ-F1,GCN4,SF1 (NR5A1)
  • recruiting TATA box binding proteins to promoters interactivators an bound
  • enhancing the DNA-binding activity of ATF1, ATF2, CREB1
  • regulating nitric oxid synthase activity by sequestering calmodulin in the cytoplasm
  • required for PPARG-stimulated adipogenesis
  • functions as a coactivator of several transcription factors
  • implicated in molecular events that are pivotal to endothelial function and, therefore, to vascular integrity
  • calmodulin binding protein that regulates calmodulin-dependent enzymes
  • inactivates the calmodulin/calcineurin/NFAT pathway via sequestration of calmodulin, during early adipogenesis
  • might have a complex role during adipose differentiation
    • CELLULAR PROCESS cell life, differentiation
    nucleotide, transcription, regulation
    PHYSIOLOGICAL PROCESS development
    text
  • repression of endothelial cells differentiation
  • angiogenesis
    • PATHWAY
    metabolism lipid/lipoprotein
    signaling
    EDF1/CALM1/PPP3CA/NFATC1 pathway
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • interacting with TBP, TFIID complex, NR5A2, NR1H3 and PPARG
  • binding NR5A1, ATF1, FOS and JUN
  • in endothelial cells, bound with CALM1, through its IQ domain sequestering this complex in the cytoplasm of endothelial cells
  • is a cofactor of PPARG
  • associates with calmodulin and calcineurin, most likely inactivating calcineurin
  • can associate in a protein complex with CALM1, and PPP3CA but not with NFATC1, suggesting that EDF1 is a key element in the CALM1/PPP3CA/NFATC1 pathway
    • cell & other
    REGULATION
    Other phosphorylated by PKA and PKC
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS