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FLASH GENE
Symbol DGAT2 contributors: mct - updated : 09-06-2015
HGNC name diacylglycerol O-acyltransferase homolog 2 (mouse)
HGNC id 16940
Location 11q13.5      Physical location : 75.479.777 - 75.512.579
Synonym name diacylglycerol O-acyltransferase-like protein 2 isoform 1
Synonym symbol(s) HMFN1045
EC.number 2.3.1.20
DNA
TYPE functioning gene
STRUCTURE 32.80 kb     8 Exon(s)
10 Kb 5' upstream gene genomic sequence study
MAPPING cloned Y linked N status provisional
Physical map
DKFZP586P0123 11q13.3 DKFZP586P0123 protein PME-1 11q13.3 protein phosphatase methylesterase-1 LOC283208 FLJ32029 KCNE3 11q13-q14 potassium voltage-gated channel, Isk-related family, member 3 HCP27 11q13.3 cytochrome c, somatic pseudogene LOC387787 11 similar to RIKEN cDNA 2610209A20 POLD3 11q14 similar to RIKEN cDNA 2610209A20 BNF1 FLJ22596 11q13.3 hypothetical protein FLJ22596 LOC387788 11 similar to ribosomal protein S12 KIAA1991 11q13.3 hypothetical protein KIAA1991 KIAA0102 11q13.3 hypothetical protein KIAA1991 LOC387789 11 LOC387789 NEU3 11q13.5 sialidase 3 (membrane sialidase) LOC390224 11 similar to olfactory receptor MOR101-2 LOC341152 11q13.3 similar to olfactory receptor MOR101-1 LOC390225 11 similar to olfactory receptor MOR101-2 SLCO2B1 11q13 solute carrier organic anion transporter family, member 2B1 ARRB1 11q13 arrestin, beta 1 RPS3 11q13.3-q13.5 ribosomal protein S3 FLJ33790 11q13.3 hypothetical protein FLJ33790 PP1665 11q13.3 hypothetical protein PP1665 SERPINH1 11q13.5 serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) MAP6 6p12-p11 microtubule-associated protein 6 MGAT2 14q21 mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase DGAT2 11q13.3 diacylglycerol O-acyltransferase homolog 2 (mouse) UVRAG 11q13 UV radiation resistance associated gene WNT11 11q13.5 wingless-type MMTV integration site family, member 11 PRKRIR 11q13.5 protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor) LOC387790 11 LOC387790 C11orf30 11q13.5 chromosome 11 open reading frame 30 GARP 11q13.5-q14 glycoprotein A repetitions predominant LOC390226 11 similar to Olfactory guanylyl cyclase GC-D precursor (Guanylate cyclase, olfactory) E2IG4 11q13.3 hypothetical protein, estradiol-induced PHCA 11q13.4 phytoceramidase, alkaline IMAGE:4907098 11q13.4 beta-1,3-N-acetylglucosaminyltransferase protein CAPN5 11q14 calpain 5 OMP 11q13 olfactory marker protein MYO7A 11q13.5 myosin VIIA (Usher syndrome 1B (autosomal recessive, severe)) LOC220032 11q13.4 GDPD domain containing protein LOC387791 11 similar to Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial 20 kDa outer membrane protein) (Outer mitochondrial membrane receptor Tom20) PAK1 11q13.5-q14.1 p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) LOC387792 11 LOC387792
RNA
TRANSCRIPTS type messenger
identificationnb exonstypebpproduct
ProteinkDaAAspecific expressionYearPubmed
8 - 2485 - 388 - 2001 11481335
- - 2336 - 345 - 2001 11481335
EXPRESSION
Type widely
   expressed in (based on citations)
organ(s)
SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
Digestiveliver    
Nervousnervespinal nervesciatic  
 nervecranial nerve  highly
Skin/Tegumentskin appendagesskin glandsebaceous gland highly
Visualeyeuveachoroid  
tissue
SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
Connectiveadiposewhite   Homo sapiens
Connectivebonesubchondral  
Epithelialbarrier/liningretinal pigment epithelium (RPE)  
Nervousperipherous   
cells
SystemCellPubmedSpeciesStageRna symbol
Blood/Hematopoieticleukocyte
cell lineage
cell lines
fluid/secretion
at STAGE
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • ER targeting signal present in the first transmembrane domain (TMD1)
  • has only two transmembrane domains and the loop connecting them is present in the ER lumen
  • HOMOLOGY
    interspecies homolog to murine Dgat2
    Homologene
    FAMILY
    CATEGORY enzyme
    SUBCELLULAR LOCALIZATION     plasma membrane
        intracellular
    intracellular,cytoplasm,organelle,mitochondria
    intracellular,cytoplasm,organelle,membrane
    intracellular,cytoplasm,organelle,endoplasmic reticulum
    intracellular,cytoplasm,cytosolic,microsome
    text
  • reside in the endoplasmic reticulum (ER), but also co-localizes with mitochondria and lipid droplets
  • also localized to near the surface of lipid droplets, where it co-localized with mitochondria
  • resides in the endoplasmic reticulum (ER), but when cells are incubated with fatty acids, DGAT2 interacts with lipid droplets presumably to catalyze localized TG synthesis for lipid droplet expansion
  • transmembrane proteins localized in the endoplasmic reticulum
  • basic FUNCTION
  • catalyzing a reaction in which diacylglycerol is covalently joined to long chain fatty acyl-CoAs, the final step in the production of triacylglycerol
  • mediates the dissociation between fatty liver and insulin resistance and this finding may be important in the prevention and treatment of insulin resistance and Type 2 diabetes in subjects with fatty liver
  • ER-resident transmembrane domain-containing enzyme, also found in mitochondria-associated membranes, where its N terminus may promote its association with mitochondria
  • DGAT1 and DGAT2 account for nearly all triacylglycerol synthesis in adipocytes and appear to be required for lipid droplet formation during adipogenesis
  • is potentially capable of catalyzing triacylglycerol synthesis and promote its storage in cytosolic lipid droplets independent of its localization in the ER
  • DGAT1 and DGAT2 can compensate for each other to synthesize triacylglycerol, but triacylglycerol synthesized by DGAT1 is preferentially channeled to oxidation, whereas DGAT2 synthesizes triacylglycerol destined for very low-density lipoprotein assembly
  • DGAT1 and DGAT2 function coordinately to regulate the process of dietary fat absorption by preferentially synthesizing triacylglycerol(TAG) for incorporation into distinct subcellular TAG pools in enterocytes
  • DGAT2 translocates to the lipid droplet (LD), associates with other proteins, and synthesizes cytosolic and luminal apolipoprotein B associated LD-triacylglycerol (TAG) from both endogenous and exogenous fatty acids
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • evolutionarily conserved SLC27A1-DGAT2 complex acts at the ER-LD interface and couples the synthesis and deposition of triglycerides into lipid droplets (LDs) both physically and functionally
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • two lipogenic genes encoding DGAT2 and FABP4 were induced in ELOVL2-overexpressing cells, whereas no such effect was seen on the fatty acid synthase (FASN) gene
  • SLC27A1 preferentially associated with DGAT2, and they acted synergistically to promote lipid droplets (LDs) expansion in mammalian cells
  • DGAT2 is regulated by AMFR-associated ERAD at the post-translational level
  • DGAT2 interacted with monoacylglycerol acyltransferase MOGAT2, an enzyme that catalyzes the synthesis of diacylglycerol, and interaction of DGAT2 and MOGAT2 serves to channel lipid substrates efficiently for TG biosynthesis
  • interacts with proteins that synthesize its fatty acyl CoA substrates
  • cell & other
  • interaction of DGAT2 with mitochondria depended on 67 N-terminal AAs of DGAT2, which are not conserved in family members that have different catalytic functions
  • REGULATION
    Other regulated in white adipose tissue by central leptin action
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    constitutional     --low  
    in psoriatic skin
    constitutional     --over  
    plays an important role in the pathogenesis of alcoholic fatty liver disease, and abnormal methionine metabolism contributes, at least partially, to DGAT2 upregulation via suppression of MEK/ERK1/2 activation
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    ANIMAL & CELL MODELS
  • mice overexpressing hepatic Dgat2 fed a high-fat diet develop fatty liver, but not insulin resistance, suggesting that DGAT2 induces a dissociation between fatty liver and insulin resistance