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FLASH GENE

Symbol

BRF1

contributors: mct - updated : 22-01-2013

HGNC name	BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae)
HGNC id	11551

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	14q32.33      Physical location : 105.675.622 - 105.767.329
Synonym name	general transcription factor IIIB, 90kD subunit
	TBP - associated factor, RNA polymerase III, 90kD
	TATA box binding protein (TBP)-associated factor 3C
	TATA box binding protein (TBP)-associated factor, RNA polymerase III, GTF3B subunit 2
Synonym symbol(s)	BRF, GTF3B, TAF3C, TAF3B2, TF3B90, TAFIII90

DNA

TYPE	functioning gene
STRUCTURE	106.29 kb     18 Exon(s)

10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

N

status

provisional

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_001519 18 splicing 3677 NP_001510 73.8 677 - 2000 10921893 involved in the transcription of tRNA adenovirus VA1,7SL and 5S RNA containing a high mobility group protein 2 (HMG2)-related region in the highly charged C terminal half of the protein and a sequence related to GTF2B in the N terminus variant 2 - splicing 2057 - 134 - 2000 10921893 required for transcription of the U6 promoter NM_145685 14 splicing 3585 NP_663718 - 473 - 2000 10921893 NM_001242786 18 splicing 3452 NP_001229715 - 584 - 2000 10921893 NM_001242787 18 - 3386 NP_001229716 - 562 - 2000 10921893 NM_001242788 17 - 3596 NP_001229717 - 650 - 2000 10921893 NM_001242789 13 - 2876 NP_001229718 - 439 - 2000 10921893 NM_001242790 - - 1170 NP_001229719 - 208 - 2000 10921893

EXPRESSION

Type

widely

expressed in	(based on citations)
organ(s)

cell lineage

cell lines

fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains

zinc finger motif

HOMOLOGY

Homologene

FAMILY

TFIIB family

CATEGORY

transcription factor

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,nucleus,nucleoplasm
	intracellular,nucleus,chromatin/chromosome

basic FUNCTION	tRNA 5S,rRNA and virus associated (VA) RNA transcription
	posttranscriptionally regulates mRNA levels by targeting AU-rich element-bearing transcripts to the decay machinery
	involved in AU-rich element-mediated mRNA decay (AMD)

CELLULAR PROCESS	nucleotide, transcription, initiation
	nucleotide, transcription, regulation

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

a component

subunit of RNA polymerase III transcription factor TFIIIB,forming a stable complex with casein kinase 2

INTERACTION

DNA

RNA

small molecule	metal binding,
	zinc Zn2+

protein	TBP (TATA-binding protein)
	MAPKAPK2 inhibits BRF1-dependent AU-rich element mRNA decay through direct phosphorylation
	HNRNPF is a co-factor in a subset of ZFP36/BRF1-mediated mRNA decay

cell & other

REGULATION

Phosphorylated by

MAPK-activated protein kinase 2 (MAPKAPK2)

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral     --other   deregulation of BRF1 and BRF2 expression could be a key mechanism responsible for the observed deregulation of RNA pol III transcription in cancer cells

Susceptibility

Variant & Polymorphism

Candidate gene

Marker

Therapy target

ANIMAL & CELL MODELS

chronic alcohol administration in mice leads to enhanced expression of Tbp, Brf1, tRNA, and 5S rRNA gene transcription in the liver