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FLASH GENE
Symbol ZNF350 contributors: mct/ - updated : 13-07-2016
HGNC name zinc finger protein 350
HGNC id 16656
DNA
TYPE functioning gene
STRUCTURE 22.54 kb     5 Exon(s)
10 Kb 5' upstream gene genomic sequence study
regulatory sequence Promoter
text structure
  • promoter contains an authentic E2F-recognition sequence that specifically binds E2F1, and promoter activity was demonstrated to be repressed upon E2F1 expression
    • MAPPING cloned Y linked N status provisional
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    5 - 2358 60 532 - 2001 11161714
    EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    physiological period pregnancy
    Text placenta
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • N terminal KRAB domain, interacting with tripartite RBCC (RING-B box-coiled coil) region, binding to the TRIM28 co-repressor (a highly conserved KRAB domain consisting of two KRAB boxes, KRAB A and KRAB B, which act generally as a transcriptional repressor domain through association with co-repressor proteins)
  • eight Kruppel-type C2H2 zinc finger motifs
  • ZBRK1-DNA binding domain acquires higher stability in the presence of zinc ions and DNA
  • C-terminal domain binds the BRCA1/RBBP8 co-repressor complex , BRCA1-binding domain, termed CTRD, which serves as a protein–protein interaction surface controlling ZNF350-directed transcriptional regulation
    • HOMOLOGY
    Homologene
    FAMILY
  • krueppel C2H2-type zinc-finger protein family
    • CATEGORY transcription factor , tumor suppressor
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,nucleus,nucleoplasm
    intracellular,nuclear envelope,matrix attachement regions
    basic FUNCTION
  • maintenance of the non dividing state of normal cells
  • transcriptional repressor that recognizes a consensus DNA element, GGGXXXCAGXXXTTT
  • involved in coordinating the cellular DNA damage response
  • co-repressor implicated in BRCA1-mediated repression of GADD45
  • acts to inhibit metastasis of cervical carcinoma, perhaps by modulating MMP9 expression
  • role in DNA damage response
  • together with the TRIM28 co-repressor, may repress other genes critical for the DNA repair process as well as other physiological functions
  • ZNF350 and LCOR form a transcriptional complex that silences gene expression, in particular FGF2, which maintains breast cell viability
  • likely suppresses renal cancer progression perhaps by regulating VHL expression
    • CELLULAR PROCESS nucleotide, transcription, regulation
    cell organization/biogenesis
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • ZNF350 forms a homotetramer in solution
  • LCOR and TRIM28 form a complex with ZNF350 on an intronic binding site in GADD45A gene and a novel site in the fibroblast growth factor 2 (FGF2) gene
    • INTERACTION
    DNA binding
    RNA
    small molecule metal binding,
  • Zn2+
    • protein
  • interacting with BRCA1 (exerts transcriptional repression through interaction with RBBP8 in the C-terminal BRCT domain and ZNF350 in the central domain)
  • ZNF589, to regulate a common set of target genes
  • interacting with MID1, tripartite RBCC (RING-B box-coiled coil) region
  • interaction partner of the SCA2 gene product ataxin-2
  • ZNF350 is a novel VHL interacting protein
  • VHL is confirmed to serve as a bridge component for the association of ZNF350 and EP300, which leads to an increase in ZNF350 transcriptional activity in the VHL promoter
    • cell & other
    REGULATION
    repressed by RB1(acts as a negative regulator of ZNF350 transcription)
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    tumoral     --low  
    in cervical tumor cells
    tumoral     --low  
    loss of ZNF350 contributes to the increased expression of TRIM28, potentiating its role to enhance metastasis and invasion in cervical cancer
    Susceptibility to breast and ovarian cancer
    Variant & Polymorphism other variants in high-risk non BRCA1/2 French Canadian breast and ovarian cancer families
    Candidate gene
    Marker
    Therapy target
    ANIMAL & CELL MODELS