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FLASH GENE

Symbol

CDC34

contributors: mct/npt - updated : 24-11-2009

HGNC name	cell division cycle 34 homolog (S. cerevisiae)
HGNC id	1734

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

DNA

TYPE	functioning gene
STRUCTURE	10.36 kb     5 Exon(s)

10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

N

status

provisional

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_004359 5 - 1462 NP_004350 - 236 - Plon (1993)

EXPRESSION

Type

widely

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Digestive liver         Endocrine pancreas       highly   thyroid       highly Nervous brain         Reproductive male system prostate       Respiratory lung         Visual eye       highly

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Epithelial secretory glandular endocrine   Epithelial secretory glandular exocrine   Lymphoid

cell lineage
cell lines	many cell lines
fluid/secretion

at STAGE

cell cycle

cell cycle, checkpoint, G1S

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains

E2 core providing specificity to CDKN1B, and the AAs 184-196 are required for possessive ubiquitination by CDC34 (Ryu 2008)

HOMOLOGY

interspecies

homolog to yeast S.cerevisiae CDC34

Homologene

FAMILY

ubiquitin conjugating enzyme family

CATEGORY

regulatory

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,cytoplasm
	intracellular,nucleus

basic FUNCTION	involved in late G1 to S transition regulator of the cell cycle
	ubiquitin conjugating enzyme E2, required for the poly ubiquitination of NFKBI
	E2-conjugating enzyme required for catalyzing the polyubiquitination reaction mediated by the Skp1.CUL1.F-box (SCF) protein E3 ubiquitin (Ub) ligase (Gazdoiu 2005)
	controls cellular proliferation through regulation of CDKN1B protein levels (Butz 2005)
	catalyzes polyubiquitination of a substrate recruited by the Skp1-Cullin 1-F-box protein-ROC1 E3 Ub ligase (Gazdoiu 2007)
	employs distinct sites to coordinate attachment of ubiquitin to a substrate and assembly of polyubiquitin chains (Gazdoiu 2007)
	plays an essential role in promoting the G1-S-phase transition of the eukaryotic cell cycle (Sadowski 2007)

CELLULAR PROCESS	cell cycle, checkpoint
	protein

PHYSIOLOGICAL PROCESS

text	ubiquitin dependent proteolysis in proteosomes

PATHWAY

metabolism

signaling

a component

component of the complex SCF (SKP1/CDC53-CUL1) /F-box rotein (SKP2), involved in the ubiquitin-proteasome degradation system

INTERACTION

DNA

RNA

small molecule

protein

cell & other

REGULATION

Other	required for polyubiquination of NFKBIA
	recruted by the complex SCF (SKP1/CDC53-CUL1) /F-box protein (SKP2)
	regulated by phosphorylation (CDC34 C-terminal tail phosphorylation regulates Skp1/cullin/F-box (SCF)-mediated ubiquitination and cell cycle progression) (Sadowski 2007)

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral     --over   in hepatocellular carcinoma

Susceptibility

Variant & Polymorphism

Candidate gene

Marker

Therapy target

ANIMAL & CELL MODELS