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FLASH GENE

Symbol

DGKA

contributors: mct/npt - updated : 30-01-2009

HGNC name	diacylglycerol kinase, alpha 80kDa
HGNC id	2849

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

DNA

TYPE	functioning gene
STRUCTURE	22.86 kb     24 Exon(s)

10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

status

confirmed

Map	cen - D1S1221E - DGKA - D12S53E - D12S1388E - qter

RNA

TRANSCRIPTS	type	messenger

text	Variants 1, 2, 3 and 4 encode the same protein

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed variant 3 24 - 2756 - 735 - Merino (2007) variant 1 24 - 2801 - 735 - Merino (2007) variant 2 25 - 2769 - 735 - Merino (2007)

EXPRESSION

Type	ubiquitous

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Digestive mouth       highly Lymphoid/Immune spleen       highly   thymus       predominantly

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Lymphoid

cells

System Cell Pubmed Species Stage Rna symbol Lymphoid/Immune lymphocyte Nervous glia

cell lineage

cell lines

fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	two N terminal EF hand calcium-binding domains
	two phorbol esters/diacylglycerol binding domain (C1 domain)
	a DAG catalytic domain
	a DAG accessory domain
	existence of additional domains that confer specific plasma membrane localization (Merino 2007)
	at least two cysteine-rich motifs of unknown function
	a conserved C-terminal domain, essential for membrane binding in a phosphatidic acid-dependent manner (Merino 2007)

mono polymer

monomer

HOMOLOGY

Homologene

FAMILY

eukaryotic diacetylglycerol kinase family

CATEGORY

enzyme

SUBCELLULAR LOCALIZATION	plasma membrane
	intracellular
	intracellular,cytoplasm,cytosolic
	intracellular,nucleus

basic FUNCTION	converting diacylglycerol to phosphatidic acid
	protein kinase C activator
	regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes (Alonso 2005)
	phosphorylates diacylglycerol to phosphatidic acid, modifying the cellular levels of these two lipid mediators (Merino 2007)
	acting as a negative modulator of diacylglycerol-based signals during T cell activation (Merino 2007)
	playing a role on the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes (Alonso 2007)

CELLULAR PROCESS

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

signal transduction

a component

INTERACTION

DNA

RNA

small molecule	metal binding,
	Ca2+

protein

cell & other

REGULATION

Other

modulating (phosphorylated) protein kinase C

ASSOCIATED DISORDERS

ANIMAL & CELL MODELS