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FLASH GENE
Symbol GTF2B contributors: mct - updated : 10-10-2015
HGNC name general transcription factor IIB
HGNC id 4648
DNA
TYPE functioning gene
STRUCTURE 38.98 kb     7 Exon(s)
Genomic sequence alignment details
10 Kb 5' upstream gene genomic sequence study
MAPPING cloned Y linked   status confirmed
RNA
TRANSCRIPTS type messenger
identificationnb exonstypebpproduct
ProteinkDaAAspecific expressionYearPubmed
7 - 1651 - 316 - -
EXPRESSION
Type ubiquitous
   expressed in (based on citations)
organ(s)
cell lineage
cell lines
fluid/secretion
at STAGE
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • a N-terminal zinc ribbon structure (TFIIB(ZR)
  • the B-finger of GTF2B is highly conserved and believed to play a role in the initiation process
  • a six-AA TIP region is needed for appropriate levels of serine 5 C-terminal domain phosphorylation and mRNA capping and for retention of the required elongation factor GTF2F1
  • two domains that engage in an intramolecular interaction that can be disrupted by the VP16 activation domain
  • C-terminal core (TFIIB(CORE)
    • HOMOLOGY
    interspecies homolog to C.elegans t04c10.3
    Homologene
    FAMILY TFIIB family
    CATEGORY transcription factor
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,nucleus,nucleoplasm
    basic FUNCTION
  • playing a major role in the activation of genes transcribed by RNA polymerase II
  • GTF2B conformational change is critical for the formation of activator-dependent transcription complexes
  • has a central role in the assembly of the preinitiation complex at the promoter, providing a platform for the entry of RNA polymerase II/TFIIF
  • plays a central role in preinitiation complex (PIC) assembly and the recruitment of RNA polymerase II (RNA pol II) to the promoter
  • only factor within the multimegadalton transcription complex that is obligatorily required to undergo dissociation and re-association with each round of mRNA transcription
  • GTF2B and GTF2F1 are both required for RNA polymerase II preinitiation complex (PIC) assembly
    • CELLULAR PROCESS nucleotide, transcription, initiation
    nucleotide, transcription, regulation
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • DAB complex with transcription factors IIB and IIA
  • component of the eukaryotic RNA polymerase II (pol II) transcription initiation machinery
  • TBP/GTF2B/Pol II complexes were linked to the production of non-polyadenylated RNA rather than polyadenylated transcripts
    • INTERACTION
    DNA
    RNA
    small molecule metal binding,
    zinc Zn2+
    protein
  • bridging GTF2D,POLR* protein
  • importance of GTF2F1 in stabilizing GTF2B interaction with the transcription complex, and potentially GTF2F1 should also participate in the process of elongation termination
  • TBP may be predominantly crosslinking to GTF2B, rather than directly to the TATA element
  • through its acidic transactivation domain (TAD), RELA has the capacity to form interactions with several different transcriptional regulatory proteins, including GTF2B, GTF2H1, EP300 and TAF9B
    • cell & other
    REGULATION
    Other phosphorylation of GTF2B is a critical event in transcription that links the gene promoter and terminator and triggers initiation by RNA pol II
    specifically and rapidly degraded by the ubiquitin-proteasome pathway
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS