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FLASH GENE

Symbol

RECK

contributors: mct/npt - updated : 23-01-2017

HGNC name	reversion-inducing-cysteine-rich protein with kazal motifs
HGNC id	11345

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

EXPRESSION

Type

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Cardiovascular heart       highly Endocrine pancreas       highly   parathyroid       highly Nervous brain       highly Reproductive female system ovary     highly   female system uterus     highly   male system testis     highly   male system prostate     highly Urinary kidney       highly

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Connective bone       Epithelial secretory glandular endocrine   Epithelial secretory glandular exocrine

cell lineage

cell lines

fluid/secretion

at STAGE

physiological period

embryo, pregnancy

Text	placenta, embryonic brain, specifically expressed in Nestin-positive neural precursor cells (Muraguchi 2007)

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	five knot repeat
	three kazal-like domains (K23 motif)
	cystein rich domain
	three protase inhibitor-like domain
	two EGFR-like domains
	five N-glycosylation sites

conjugated

GlycoP , LipoP

mono polymer

heteromer , complex

isoforms

Precursor

a 26 aa signal peptide of 2.5 kda, a 945 aa mature peptide of 104 kda

HOMOLOGY

interspecies	homolog to rattus Reck (92.4 pc)
	homolog to murine Reck (93.1 pc)

Homologene

FAMILY

CATEGORY

regulatory , tumor suppressor

SUBCELLULAR LOCALIZATION	extracellular
	plasma membrane
text	lipid-anchor
	GPI-anchor

basic FUNCTION	acting as a negative regulator for matrix metalloproteinase 9 and 2 (MMP9, MMP2)
	inhibiting tumor invasion
	acting as a serine-type endopeptidase inhibitor
	regulates the ectodomain shedding of Notch ligands by directly inhibiting the proteolytic activity of ADAM10
	inhibitor of ADAM10, an upstream regulator of Notch signaling and a critical modulator of brain development (Muraguchi 2007)
	inhibits the enzymatic activities of some matrix metalloproteinases (MMP), thereby suppressing tumor cell metastasis (Takagi 2009)
	critically regulates Notch signaling by antagonizing the ectodomain shedding of Notch ligands, which is mediated by a disintegrin and metalloproteinase domain 10 (ADAM10) (Muraguchi 2007)
	GDE2 promotes neurogenesis by glycosylphosphatidylinositol-anchor cleavage of RECK

CELLULAR PROCESS	cell cycle, progression

PHYSIOLOGICAL PROCESS

text	negative regulation of progression through cell cycle

PATHWAY

metabolism

signaling

a component	N-glycosylated
	forming a complex with MT1-MMP

INTERACTION

DNA

RNA

small molecule

protein	interacting with MMP9 (negative regulator of MMP9 transcription) (Takagi 2009)
	interacting with Tgat oncoprotein
	cleaved by MMP2 and MMP7 and competitively inhibits MMP-7-catalyzed cleavage of fibronectin (Omura 2009)
	RECK and GPR124 are part of the cell surface protein complex that transduces WNT7A- and WNT7B-specific signals in mammalian CNS endothelial cells (ECs) to promote angiogenesis and regulate the Blood-Brain Barrier

cell & other

REGULATION

inhibited by

Tgat oncoprotein

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral     --low   downregulation caused by promoter methylation in non-small cell lung cancer

Susceptibility

Variant & Polymorphism

Candidate gene
Marker
Therapy target	K23 motifs of RECK protein can inhibit MMP9 secretion and activity and attenuate metastasis of lung cancer cells (Chang 2008)

ANIMAL & CELL MODELS