10 KB upstream the 5' end of the gene( Copy and paste the sequence to use a program below, blastn and mask excepted)
Mask the 5' upstream region with RepeatMasker Mask  
Promoter prediction BDGB : Neural Promotor Prediction
BIMAS : Promotor Scan
CBS : Promotor prediction V2.0
MIT : MC Promoter MM. II
CpG islands prediction EBI : CpGplot
Signal prediction BIMAS : Signal Scan
Blast 10kb upstream human, mouse, rat genes database (more sensitive with masked sequence)