-------------------------------------------------------------------------------- GrailEXP v3.31 [March, 2002] http://compbio.ornl.gov/grailexp/ Authors: Doug Hyatt, Manesh Shah, Victor Olman, Richard Mural, Ying Xu, and Edward C. Uberbacher, 1996-2001 Reference: "Automated Gene Identification in Large-Scale Genomic Sequences", Xu, Y. and Uberbacher, E.C., Journal of Computational Biology, Volume 4, Number 3, 1997 Sequence: >GrailEXP Input Sequence (45677 bp) -------------------------------------------------------------------------------- PERCEVAL Exon Candidates (21 predicted) Index Std Begin End Frm Type Len Scr Quality Exon 1 + 1263 1322 0 Initial 60 61 Marginal gcccacctcacctcctcgcctatcgttagttctctgcgaaaccgcgaactgggggcgcgg cctatcttcagccccgcccctgatgggcctatacaagggggcggttccgcgcgcgcccgc ccagttggagccagacagcggggtggacaagtggcgtgtgtgctgcgaccccgagggaag ATGAACGGGACGCGGAACTGGTGTACCCTGGTGGACGTGCACCCAGAGGACCAGGCGGCG gtaagaaaaagcgctctcgctgtcttctccgttttgtattcccggtttctaagtccgccc cttccgtgccccgcgccgtgttggctgacatcacctggtgcccggctcgcgcggtcagcc ctgcgcggcgcttctctctttttattcttttgcctgcgacgggcagtgggagtcgcgggg Exon 2 + 3012 3036 2 Terminal 25 91 Excellent gaactcctggcctcaatccttctgcctcagcctcctgagtagctgggacttcagatgcac accacctatagcttctcttccatgctcttgctctctcttttggaatagttgagggaaaag ggtatgagataaataaaggagagggatgtttgggctgctttttgcttgcttgctatatag AAAAGAGATCCGGAAAGAAGTCTAA gaccaagttggcactggcagtgttaatgaatcattaacctctactattttttttctttct tcctttcttttcttttctcttttcttttctctttttttgagacagggccttgctctgttg cccaggctggagtgcaatggcatgatctcagctcactgcaacctccacctcctggtttca Exon 3 + 5538 5571 2 Terminal 34 65 Marginal aatttttgtacttttactagagatggggtttcatcatgttggccaggctggtctcaaact cctgacctcaaatgatctgcccgcttcggccttccaaagtactgggattacaggcgtgag ccaccgtgcctggcctcttaaatcactttttatcaaaggagtttcactcttgttgcccag GCTGGAGTGCAACGACGTGATCTCGGACATTTAA aatcacatatgtgcatgattttaaagtatcaaagtatcaataatatcaaaaggcttttaa aagaaaatagcagtcccctgctccaagttctgctccatggaagtaaccactttctactct ttgagctgttttgtgtttacctccatgtttctaaataatatgcttacagtgattttaatt Exon 4 + 16766 16878 1 Terminal 113 94 Excellent tcgtttgaacccaggaggcagaggttgcagtgagctgagatggtgccattgcactccagc ctgggtgacaaggtgagactccgtctcaaaaaaaaaaaaaaaaaaaagataattatagat tcacatgcagttataagaaataatagaattcccccatatcctttattccattttttccag TGGTAACATCTTGCAAAACTGTGGTACTCTATCACAACCAGGATATTGGCATTGATACAG TCAAGCTATGGAACATTTCCATCACCAAAAGCACCCCTCATGTTGCCCTTTGA gagccacactcatttccctcctcccccccactttcttcttaactcctagtaaccaccagt ccttttcccatttctataactttgttatttaaaaaatgttacacaagtgaaattatagag catgtaaacttttgtgattggcttttttcactcagcaaattctctggagagtgttgttgc Exon 5 + 18342 18665 0 Single 324 92 Excellent ttctcccatttatgatactaaatatttaatagtataatattatcatatttaaatattttc tctgcatacatttagaaccacatcagacattgttatactttttgctttaatcatcaaatg taacttagaaaacctaagaggggctttccagccccagccccggcccctacagccaccgag ATGTTGATGCCTAAGAACCGGATTGCCATTTATGAACTCCTTTTTAAGGAGGGAGTCATG GTGGCCAAGAAGGATGTCCACATGCCTAAGCACCGGGAGCTGGCAGACAAGGATGTGCCC AATCTTCATGTCATGAAGGCCATGCAGCCTCTCAAGTCCCGAGGCTACGTGAAGGAACAG TTTGCCTGGAGACATTTCCACTGGTACGTTACCAATGAGGGCATCCAGTATCTCCGTGAT TACCTTCATCTGCCCCGGAGATTGTGCCTGCCACTCTATGCTGCAGCCGTCCAGAGACTG GAAGTCCTCAGCCTAAAGGTCTGA agggtgagcgacctgcaagactcacaagagggaaagcggacagagataacctacagacgg agtgctgtgccgcctggtgccgacagcaaagctgaggctggggctgggtcagcaaccgaa ttccagtttagaggtggatttggttgaggatgtggtcagccacctcagtaaaattggaga Exon 6 + 20283 20349 0 Initial 67 59 Marginal tggagcattctctgtgcattcagaaaatacctttgctaagggctgtggcatacaacagct cttgaggagttaacagaaggggtaattcttcacaaaacacaaataactgagaggcagatg ccatgggaggccttgtgacgctgtgggagctcaaggaggggaagatgtgtttcccacagg ATGCGGGGTAGCATCTTGGAAGATGAGGCATTTGAACATGAACTTGAGGAACTTGGAGAC GCCTTAG gtgaaaatgggaagaatagatatttaaggcacaggaaatgatgggagcaaaggcactgaa gttggaaaccatggaatgtattggatagggcactgagagatggtagcaagaggtgtgagt ttacccagactgtggaaagccttgcatgccactctaaggatgtggcttttatttcatagg Exon 7 + 23481 23582 0 Internal 102 100 Excellent ctgtgtggcttctgttagaagttcggaaacgaatttttggagaggaaagtcagactaatc cttcaagtcttttgagaaacaggtcttctgcattagctgaggggcaggagttctgggctt gccatgtggtgtctgagccatctctctctctctctctctctctcatctctctttggccag GCGGGCAGGAAGACCTATGCCATGGTGTCCAGCCACTCAGCTGGTCATTCTCTGGCTTCA GAACTGGTGGAGTCCCATGATGGACATGAGGAGATCATTAAG gtaagcttagttcacctccaacttttctccctgtcctcttcctccctgcacgtatatgag aaggagcagttgatgttgttgccaaagggagcttgtggtttgatggaaggaaccagacct gagctgctagttcctggcaatctcctagaaatatcttaattaataccaggagactacagt Exon 8 + 24800 24886 0 Internal 87 100 Excellent agtagaactgatagaccaaaaagtaaatatatttttaggtcttctgatccgaatcaccaa attccacccagtaaattggtggtaacaatttaccctcccaccagcactatatgaagctgc ctatttttgtatagttaatttaatttgaggtttcttaactaaatggtgttcttatttcag GTGTACTTGAAGGGGAGGTCTGGAGACAAGATGATTCACGAGAAGAATATTAACCAGCTG AAGAGTGAGGTCCAGTACATCCAGGAG gtgggcaccccttacctctcacgcagtgcctaggtcatttctctaaatctcacatttttt gtgactgtcttcttttgtggattctggtttttccccttctttttcctttatgtctctgaa atgccaccatgccctcccgtgtgtcaaatatataacttttgaccatacctgatccattaa Exon 9 + 25421 25550 0 Internal 130 51 Marginal ccccaacaagaatgtcagctgtagctccacttgaccaaggtccctccctccatcctgcag ccagtcgttaggacagtgcctgactcgcagtacaggccagggatggggcacagagttggg tgagttggcatgctctctttctctaccctaaagctcaagtttcttcccctcccctcccag GCCAGGAACTGCCTACAGAAGCTCCGGGAGGATATAAGTAGCAAGCTTGACAGGAACCTA GGAGATTCTCTCCATCGACAGGAGATACAGGTAATAGGAAATGGTCCATGGTTGGGTCCC CACCGAGGAG gtggggttgtgccaggcagtctcatttaccaattacccagtgtgtgctaaatctagcatc cctgccaggaatggagaaaatagaggtgatgctggcttctctggagcactcacaatctgg ttaggactctccagccgatgagcacttacatttacacatgtcagcttaaacataagtaca Exon 10 + 27059 27124 0 Internal 66 70 Good tttcttcattccttcctttttctccctccctttctttttctctctccctccccctccctt tcaccttcctctccctctccctgtccttctctttctaagatttgagtgttaggagaataa tgtttcaaagttggttgctttcatctgttttaactaccaattttatttttctttgaacag GTGGTGCTAGAAAAGCCAAATGGCTTTAGTCAGAGTCCCACAGCCCTGTACAGCAGCCCA CCTGAG gtaggtaacagaggacaccatggtggctccgcttagccaggggcaggattgcctcctccc catccttttgtttattgctttctctctctctctctctctcattaaatacaagagtagtag ttgacataaaaattcaaacacagagaaatacaaagagtagaaattctccttccccaatct Exon 11 + 30553 30666 0 Internal 114 100 Excellent attgaataactcagaattcctggctgcctaaggtaactctccccttgtgtgatgacagtt cttttttgagggtggtctggacgagatggtccaaaccagtgtgtgcctgggaggggggac cttttcttactgagtcagaaacttgagggcttccccttcccttccttgttctggctgtag GTGGACACCTGTATAAATGAGGATGTTGAGAGCTTGAGGAAGACGGTGCAGGACTTGCTG GCCAAGCTTCAGGAGGCCAAGCGGCAACACCAGTCAGACTGTGTGGCTTTTGAG gtgagatttgggatttggggagaaggtgccctaagggatgggctactgaacacaggtgcc agtgatgtctcagcttggacttcctgtctttctggctgagtgccacaaactggaaaggca aggctgtgaagcctggaacgcttgtctgcttttccctctcctggacgtttacactgttta Exon 12 + 33546 33646 0 Initial 101 69 Marginal tgtacagaaatgtacataaataaacataaataaatgtacataaataaaatttttttaaaa ggccttacttttaagttcctgcctatggggatggcctatttcaggttaatatgggtcata tgaaggcttgtctcagtctctcctagtgaattggactccttggtctacttttctgaacct ATGGGTAGGAAAGGCAAGATGATCAATTACCATGAAAATAACTTGCCATTAAGCATGGGT TTCCTGTTCTACTGGCTGCTTGCTGTCAAGAAGGATTTGCA gtgagagtgagtttgctctggacatggctggtgtgtagcatggacacgtcccatcacaca tcacctgtgccgtactttctcctccccagttctgaccttgggaggggctggacgaactgg ctgctgatgttctgttttcccagggctggctgctaggctttccagaagttacctggattc Exon 13 + 35166 35294 0 Internal 129 90 Excellent ctggagtgctgaggtggtcctgtgcagtgaaggggaggtggtggtgagcccatcagtgtc accacctttctcccgtcatggctccagagccgcccaggttcctgggttcttttctaaggt ttttttccatgtttgctcttttttccacaccatttaacatattcctgctccgtgttttag GTCACACTCAGCCGGTACCAGAGGGAAGCAGAACAAAGTAATGTGGCCCTTCAGAGAGAG GAGGACAGAGTGGAGCAGAAAGAGGCAGAAGTCGGAGAGCTGCAGAGGCGCTTGCTAGGG ATGGAGACG gtaaccgggggatcttgcttgtcagtgcctggactcctgggctggaaggggcctgcaggt ggatcgaaacggttgccgagacccttcttttccaagcctctccagaaaagcacacttcgc accttcctttggcagatcccttggaatacattttagccttcaaggaattagcctccccct Exon 14 + 35807 35901 0 Internal 95 100 Excellent agagtgggaagggttctcctcatcaagggatggctccccatcaaaccagctcatcttgct gctctttggcagctgtccaggcccagtaggggctgagtcccagcctggcgcccatcttag cccaaacgcagaaactgttgccatcttctcatcctagattttcttttgtggcccacccag GAGCATCAGGCCTTACTGGCGAAAGTGAGGGAAGGGGAGGTGGCCCTAGAGGAACTTCGG AGCAACAATGCTGACTGCCAAGCAGAACGAGAAAA gtaagggcttggctcttgttcacggtgaagttgggttgcagggaggggagggcaggccat acagcctgttgctcctgtggtggcctggtctggggaggaagctggtgctgatgtcagatc ttccagctctgactcaccgtgagcttgctcagggtggagccccacccaagagataccatt Exon 15 + 38269 38395 2 Terminal 127 48 Marginal gaacccctttgtttagggttcttgtggcggttcattacattggcatgattgattaaatta ttggccactggtgattagtttagtctctagctcctatcccctgtccctttcccagaggct agggggagttgtggggctgaaagtttcaaccctttaatcatgccttggtcttttcactag CCCCATCCTGAAGCTATCTAGGGACCCCAGCCACCAGTCATCATTAGCATACAAAAAGAC ACTCATCAGTCTTGGAGATGCAAAGGCTCAGGAACCAGGGAATGTGACCAAATATAACAA AACATAG gtgctgtatgtcaggaaccgggacagagacccattatatatttcttattattttacactg ccctttgggggatcttggctcagtttgcttagaagatgcaaaggaagcaacagtggtttg ggaatcaggagacctagaaacaaaattgtgtgaagaggctttaaggagcctcaaattctg Exon 16 + 39617 39722 2 Internal 106 100 Excellent ctcagtgcttccaatgtgatattctaaggtacccaccacctactcttcagagcagcctac cagggaatcaggcaaagggaatcatattaatgggaacctcccaagctcaaggctcttcct gtgactgggtaaataagtgatcttgttccattgcctcagccttctggtctttatccccag GGCTGCTACCCTGGAAAAGGAAGTGGCCGGGTTGCGGGAGAAGATCCACCACTTGGATGA CATGCTCAAGAGCCAGCAGCGGAAAGTCCGGCAAATGATAGAGCAG gtaagttgggctggtttgtaacctgccctcggggagtcaccccaagtctatgctcttatt tgtttcctagggagcttgacttatattcatgcatttcccatgtcaaacatttatagctac caattaccaggttcaggttattaggatttgcttttctcccatctttcccctactgttttg Exon 17 + 40545 40628 0 Internal 84 96 Excellent tcccacccctgggataatcaagagtctaatattgactgaaaggaggcctttgtagaaaag ctaatgaagtcatatggagttgcagggctctgctgccttccccagcagctggggtgaagt ctggtgagtgtgtaatgagtcattgcaaaatgagctccagtgtctcccacacctttgcag CTCCAGAATTCAAAAGCTGTGATCCAGTCAAAGGACGCCACCATCCAGGAGCTCAAGGAG AAAATCGCCTATCTGGAGGCAGAG gtgtgtgtgctgggcagcccagcaggggaacaggactcttgaagagaaggtggacataag ggagacagaggttatgggaggggtctttgtcatgtcttgctgttgctctgaagtgaatac ctagggtggttggttgactttcagttcagctgaataaacattttctgagaaccttcagga Exon 18 + 40807 40871 0 Internal 65 61 Marginal aggtgtgtgtgctgggcagcccagcaggggaacaggactcttgaagagaaggtggacata agggagacagaggttatgggaggggtctttgtcatgtcttgctgttgctctgaagtgaat acctagggtggttggttgactttcagttcagctgaataaacattttctgagaaccttcag GAAGCTGCTAACAATGATGAGATGACCACCCCCCTGGCCCCACCTTCAACGGACATGTCA TCATG gtgcaagccacatgccatggctgcacacggctaattctgaccctccaggctttgcaaagg agctggcagtggtgctgggctgtgaaggaggaggaggcattcatacagatggagcctttt ccagggctagattacgtaacgagttgccttcctagtctaatttccactttgccacagggc Exon 19 + 41049 41113 1 Terminal 65 87 Good atggtgcaagccacatgccatggctgcacacggctaattctgaccctccaggctttgcaa aggagctggcagtggtgctgggctgtgaaggaggaggaggcattcatacagatggagcct tttccagggctagattacgtaacgagttgccttcctagtctaatttccactttgccacag GGCCCTACCTGTCTGTTGACTACCTGAAGATCACTTTGCGAACGCTCTGTGAAGATCCCC TGTAG tgagggagaaaaatgggagaaagggttatctttagagagtttgcagtctatagtcagaga taaaatgtgtatttttaaaaagaccaaagaatgctttatagaatagcgtatcaacaaagg caagtgagtatctcaactgaggacaaaaaattatatataggggccaggtgcagtggctca Exon 20 + 41847 41938 0 Initial 92 83 Good aatctatggtaatgaagatgaggcaagtgggtatccaggtggtggcacgcatggtggggg tagaggcaacagcagcataaacacaggcagagagaccagaatgcactagctgaacgtggc acccgggaagccagaaaaaggccaactctaacccctaagcttgtgttacagaatttagag ATGCATGACCGGATGGAACACCTGATAGAAAAACAAATCAGTCATGGCAACTTCAGCACC CAGGCCCGGGCCAAGACAGAGAACCCGGGCAG gtgagtgagcgtgtgtagatagaatggggccagggaggaggggagaaggagggacagagc tctgtagaacctttagtgctaaggcaagaacaaggctcctttaaatccaactttcctccc tcagtctgacccactgtttgccggaaccattttctagcccaattagagtagaacctgtta Exon 21 + 44730 44819 2 Internal 90 63 Marginal tgggcaccctgttatccacatgtgggaggtgtggtccaaacatcccaaatgccatgatat gaggactttgtggcatttatagaggtattagaagaagctcacctccgtgctctgagtcgt gattgtaggaggcagcccctaactttagctttgaccctctctggagcaatggccttgtag GATCCCTAAGCCTGGCCGCATTGTGTCTCATTTGGAAGAGATAGAGGTAGAGTCCTGTGA TTACATAGAGATAGAACAGCAGCACTGGAG gtagatgagtaactgcatgccttctgcagaaatgaaaagcactctggaaatgctgactgc agctgttcaagggggttcgtttctggctgtctctgggcatcattcaattagtctcatgat tttttttttcctttctgccaagaaggcagttgactgcagggccaaatggggacatagctg --------------------------------------------------------------------------------